Chemogenetic manipulation of parasympathetic neurons (DMV) regulates feeding behavior and energy metabolism

Cherl NamKoong, Woo Jin Song, Chang Yeon Kim, Duck Hyeon Chun, Soonho Shin, Jong Woo Sohn, Hyung Jin Choi

Research output: Contribution to journalArticle

Abstract

Parasympathetic nervous system (PNS) innervates with several peripheral organs such as liver, pancreas and regulates energy metabolism. However, the direct role of PNS on food intake has been poorly understood. In the present study, we investigated the role of parasympathetic nervous system in regulation of feeding by chemogenetic methods. Adeno associated virus carrying DREADD (designer receptors exclusively activated by designer drugs) infused into the target brain region by stereotaxic surgery. The stimulatory hM3Dq or inhibitory hM4Di DREADD was over-expressed in selective population of dorsal motor nucleus of the vagus (DMV) neurons by Cre-recombinase-dependent manners. Activation of parasympathetic neuron by intraperitoneal injection of the M3-muscarinic receptor ligand clozapine-N-oxide (CNO) (1 mg/kg) suppressed food intake and resulted in body weight loss in ChAT-Cre mice. Parasympathetic neurons activation resulted in improved glucose tolerance while inhibition of the neurons resulted in impaired glucose tolerance. Stimulation of parasympathetic nervous system by injection of CNO (1 mg/kg) increased oxygen consumption and energy expenditure. Within the hypothalamus, in the arcuate nucleus (ARC) changed AGRP/POMC neurons. These results suggest that direct activation of parasympathetic nervous system decreases food intake and body weight with improved glucose tolerance.

Original languageEnglish
Article number134356
JournalNeuroscience Letters
Volume712
DOIs
StatePublished - 1 Nov 2019

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Parasympathetic Nervous System
Motor Neurons
Feeding Behavior
Energy Metabolism
Neurons
Designer Drugs
Eating
Body Weight
Muscarinic M3 Receptors
Feeding Methods
Glucose
Pro-Opiomelanocortin
Dependovirus
Arcuate Nucleus of Hypothalamus
Glucose Intolerance
Intraperitoneal Injections
Oxygen Consumption
Hypothalamus
Weight Loss
Pancreas

Keywords

  • DREADD, CNO
  • Energy expenditure.
  • Food intake
  • PNS

Cite this

NamKoong, Cherl ; Song, Woo Jin ; Kim, Chang Yeon ; Chun, Duck Hyeon ; Shin, Soonho ; Sohn, Jong Woo ; Choi, Hyung Jin. / Chemogenetic manipulation of parasympathetic neurons (DMV) regulates feeding behavior and energy metabolism. In: Neuroscience Letters. 2019 ; Vol. 712.
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Chemogenetic manipulation of parasympathetic neurons (DMV) regulates feeding behavior and energy metabolism. / NamKoong, Cherl; Song, Woo Jin; Kim, Chang Yeon; Chun, Duck Hyeon; Shin, Soonho; Sohn, Jong Woo; Choi, Hyung Jin.

In: Neuroscience Letters, Vol. 712, 134356, 01.11.2019.

Research output: Contribution to journalArticle

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