Change of hepatitis B virus DNA status in anti-HBc positive liver graft.

Choon Hyuck Kwon, Kyung Suk Suh, Jai Young Cho, Nam Joon Yi, Ja June Jang, Kuhn Uk Lee

Research output: Contribution to journalArticle

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Abstract

BACKGROUND/AIMS: Many patients with positive anti-HBc, but negative HBsAg, are known to harbor occult HBV infection, which may transmit the virus through the graft in liver transplantation. We examined the change of HBV DNA within the liver allograft tissue of the donor with positive anti-HBc, but negative HBsAg, before and after the transplantation and assessed its significance. METHODS: Twenty-eight patients with available posttransplant biopsies that received anti-HBc positive liver allografts between April 2000 and November 2003 were enrolled in the study. Intraoperative wedge biopsy of donor liver and needle biopsy of the recipient around the 12th postoperative day were used. HBV DNA within the liver tissue was identified by polymerase chain reaction technique using paraffin-embedded liver tissue. RESULTS: Among 13 patients that showed positive amplification before transplantation, 10 turned negative and 3 remained positive after transplantation. One patient, who was negative, became positive after transplantation. Three patients had recurrent HBV infection, but none had positive PCR before or after transplantation and recurrence was not associated with PCR results. Donors with low anti-HBs titer were more likely to be PCR positive compared to donors with high anti-HBs serology (P<0.05). CONCLUSIONS: Under adequate prophylactic measures, the presence of HBV DNA within the liver tissue does not affect recurrence and most allografts harboring HBV DNA before transplantation will eventually show viral clearance. However, many anti-HBc positive allografts are infected by HBV at subclinical level so vigilant surveillance is essential.

Original languageEnglish
Pages (from-to)191-200
Number of pages10
JournalThe Korean journal of hepatology
Volume12
Issue number2
StatePublished - Jun 2006

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Hepatitis B virus
Transplantation
Transplants
Allografts
Liver
DNA
Tissue Donors
Polymerase Chain Reaction
Hepatitis B Surface Antigens
Biopsy
Recurrence
Needle Biopsy
Serology
Infection
Liver Transplantation
Paraffin
Viruses

Cite this

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title = "Change of hepatitis B virus DNA status in anti-HBc positive liver graft.",
abstract = "BACKGROUND/AIMS: Many patients with positive anti-HBc, but negative HBsAg, are known to harbor occult HBV infection, which may transmit the virus through the graft in liver transplantation. We examined the change of HBV DNA within the liver allograft tissue of the donor with positive anti-HBc, but negative HBsAg, before and after the transplantation and assessed its significance. METHODS: Twenty-eight patients with available posttransplant biopsies that received anti-HBc positive liver allografts between April 2000 and November 2003 were enrolled in the study. Intraoperative wedge biopsy of donor liver and needle biopsy of the recipient around the 12th postoperative day were used. HBV DNA within the liver tissue was identified by polymerase chain reaction technique using paraffin-embedded liver tissue. RESULTS: Among 13 patients that showed positive amplification before transplantation, 10 turned negative and 3 remained positive after transplantation. One patient, who was negative, became positive after transplantation. Three patients had recurrent HBV infection, but none had positive PCR before or after transplantation and recurrence was not associated with PCR results. Donors with low anti-HBs titer were more likely to be PCR positive compared to donors with high anti-HBs serology (P<0.05). CONCLUSIONS: Under adequate prophylactic measures, the presence of HBV DNA within the liver tissue does not affect recurrence and most allografts harboring HBV DNA before transplantation will eventually show viral clearance. However, many anti-HBc positive allografts are infected by HBV at subclinical level so vigilant surveillance is essential.",
author = "Kwon, {Choon Hyuck} and Suh, {Kyung Suk} and Cho, {Jai Young} and Yi, {Nam Joon} and Jang, {Ja June} and Lee, {Kuhn Uk}",
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Change of hepatitis B virus DNA status in anti-HBc positive liver graft. / Kwon, Choon Hyuck; Suh, Kyung Suk; Cho, Jai Young; Yi, Nam Joon; Jang, Ja June; Lee, Kuhn Uk.

In: The Korean journal of hepatology, Vol. 12, No. 2, 06.2006, p. 191-200.

Research output: Contribution to journalArticle

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AU - Jang, Ja June

AU - Lee, Kuhn Uk

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