Ceramide PC102 inhibits melanin synthesis via proteasomal degradation of microphthalmia-associated transcription factor and tyrosinase

Hyo Soon Jeong, Hye Ryung Choi, Hye Young Yun, Kwang Jin Baek, Nyoun Soo Kwon, Kyungchan Park, Dong Seok Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A few types of ceramide are reported to decrease melanin synthesis. In the present study, we examined the effects of an artificial ceramide analog, PC102, on melanogenesis using a spontaneously immortalized melanocyte cell line (Mel-Ab). PC102 is currently used as a moisturizing additive in a variety of cosmetics. Our data showed that PC102 inhibited melanin production and tyrosinase activity in a dose-dependent manner, but did not directly affect tyrosinase activity. Microphthalmia-associated transcription factor (MITF), tyrosinase, and β-catenin protein levels decreased after 48 h of PC102 treatment. In contrast, PC102 did not decrease MITF, tyrosinase, and β-catenin mRNA levels. Therefore, we investigated whether the decrease in MITF and tyrosinase by PC102 is due to proteasomal degradation. MG132, a proteasomal inhibitor, completely abolished tyrosinase downregulation due to PC102 and partially reduced the downregulation of MITF and β-catenin due to PC102. Moreover, MG132 abrogated the inhibition of melanin synthesis by PC102. Taken together, our data suggest that PC102 may inhibit melanin synthesis through MITF and tyrosinase degradation.

Original languageEnglish
Pages (from-to)81-87
Number of pages7
JournalMolecular and Cellular Biochemistry
Volume375
Issue number1-2
DOIs
StatePublished - 1 Mar 2013

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Microphthalmia-Associated Transcription Factor
Monophenol Monooxygenase
Ceramides
Melanins
Degradation
Catenins
Down-Regulation
1,3-bis(N-2-(hydroxyethyl)lauroylamino)-2-hydroxypropane
Cosmetics
Melanocytes
Corrosion inhibitors

Keywords

  • Ceramide PC102
  • MITF
  • Melanin
  • Tyrosinase

Cite this

Jeong, Hyo Soon ; Choi, Hye Ryung ; Yun, Hye Young ; Baek, Kwang Jin ; Kwon, Nyoun Soo ; Park, Kyungchan ; Kim, Dong Seok. / Ceramide PC102 inhibits melanin synthesis via proteasomal degradation of microphthalmia-associated transcription factor and tyrosinase. In: Molecular and Cellular Biochemistry. 2013 ; Vol. 375, No. 1-2. pp. 81-87.
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Ceramide PC102 inhibits melanin synthesis via proteasomal degradation of microphthalmia-associated transcription factor and tyrosinase. / Jeong, Hyo Soon; Choi, Hye Ryung; Yun, Hye Young; Baek, Kwang Jin; Kwon, Nyoun Soo; Park, Kyungchan; Kim, Dong Seok.

In: Molecular and Cellular Biochemistry, Vol. 375, No. 1-2, 01.03.2013, p. 81-87.

Research output: Contribution to journalArticle

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