CD8+ T Cells Require ITK-Mediated TCR Signaling for Migration to the Intestine

Hyoung Soo Cho, Soyoung Ha, Hyun Mu Shin, Andrea Reboldi, Jason A. Hall, Jun R. Huh, Edward J. Usherwood, Leslie J. Berg

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The Tec kinase IL-2–inducible T cell kinase (ITK) regulates the expression of TCR-induced genes. Itk2/2 T cell responses are impaired but not absent. ITK inhibition prevented colitis disease progression and impaired T cell migration to the colon in mice. To examine the function of ITK in T cell migration to the intestine, we examined the number of gut T cells in Itk2/2 mice and then evaluated their expression of gut-homing receptors. Combined with in vitro murine T cell stimulation and in vivo migration assay using congenic B6 mice, we demonstrated an essential role for ITK in T cell migration to the intestine in mice. Reconstitution of Itk2/2 mouse CD8+ T cells with IFN regulatory factor 4 restored gut-homing properties, providing mechanistic insight into the function of ITK-mediated signaling in CD8+ T cell migration to the intestinal mucosa in mice.

Original languageEnglish
Pages (from-to)57-71
Number of pages15
JournalImmunoHorizons
Volume4
Issue number2
DOIs
StatePublished - 1 Feb 2020
Externally publishedYes

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