CD39-mediated effect of human bone marrow-derived mesenchymal stem cells on the human Th17 cell function

Jong Joo Lee, Hyun Jeong Jeong, Mee Kum Kim, Won Ryang Wee, Won Woo Lee, Seung U. Kim, Changmin Sung, Yung Hun Yang

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35 Scopus citations

Abstract

This study investigated the immune-modulatory effects of human bone marrow-derived mesenchymal stem cells (hBMSCs) on human Th17 cell function through the CD39-mediated adenosine-producing pathway. The suppressive effects of hBMSCs were evaluated by assessing their effects on the proliferation of Th17 cells and the secretion of interferon (IFN)-γ and interleukin (IL)-17A by Th17 cells with or without anti-CD39 treatment. Changes in CD39 and CD73 expression on the T cells with or without co-culture of hBMSCs were evaluated by flow cytometry. hBMSCs effectively suppressed the proliferation of Th17 cells and the secretion of both IL-17A and IFN-γ from Th17 cells using by both flow cytometry and ELISA, while anti-CD39 treatment significantly reduced the inhibitory effects of hBMSCs on the proliferation and secretion of the Th17 cells. The hBMSCs induced increased expression of the CD39 and CD73 on T cells correlated with the suppressive function of hBMSCs, which was accompanied by increased adenosine production. Our data suggests that hBMSCs can effectively suppress immune responses of the Th17 cells via the CD39-CD73-mediated adenosine-producing pathway.

Original languageEnglish
Pages (from-to)357-365
Number of pages9
JournalPurinergic Signalling
Volume10
Issue number2
DOIs
StatePublished - Jun 2014

Bibliographical note

Funding Information:
Acknowledgments This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (MEST) (NRF 2010-0010629 and NRF 2011-0004128)

Keywords

  • Adenosine
  • Bone marrow-derived mesenchymal stem cells
  • CD39
  • CD73
  • Interferon-γ
  • Interleukin-17
  • Th17 cells

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