CD19 signalling improves the Epstein-Barr virus-induced immortalization of human B cell

D. Y. Hur, M. H. Lee, J. W. Kim, J. H. Kim, Y. K. Shin, J. K. Rho, K. B. Kwack, Wang Jae Lee, Bok Ghee Han

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Abstract

Epstein-Barr virus (EBV) infection in vitro immortalizes primary B cells and generates B lymphoblastoid cell lines (LCLs). These EBV-LCLs have been used for several purposes in immunological and genetic studies, but some trials involving these transformations fail for unknown reasons, and several EBV-LCLs do not grow in normal culture. In this study, we improved the immortalization method by CD19 and B-cell receptor (BCR) co-ligation. This method shortens the time required for the immortalization and generation of EBV-LCLs but does not alter the cell phenotype of the LCLs nor the expression of the EBV genes. In particular, the CD19 and BCR coligation method was found to be the most effective method examined. EBV-infected B cells induced by CD 9 and/or BCR ligation expressed the intracellular latent membrane protein LMP-1 earlier than EBV-infected B cells, and the expression of intracellular LMP-1 was found to be closely related to the time of immortalization. These results suggest that the modified method, using CD19 and/or BCR ligation, may efficiently generate EBV-LCLs, by expressing intracellular LMP-1 at an early stage.

Original languageEnglish
Pages (from-to)35-45
Number of pages11
JournalCell Proliferation
Volume38
Issue number1
DOIs
StatePublished - 1 Feb 2005

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Human Herpesvirus 4
B-Lymphocytes
Cell Line
Ligation
Intracellular Membranes
Epstein-Barr Virus Infections
Membrane Proteins
Phenotype
Genes

Cite this

Hur, D. Y., Lee, M. H., Kim, J. W., Kim, J. H., Shin, Y. K., Rho, J. K., ... Han, B. G. (2005). CD19 signalling improves the Epstein-Barr virus-induced immortalization of human B cell. Cell Proliferation, 38(1), 35-45. https://doi.org/10.1111/j.1365-2184.2005.00328.x
Hur, D. Y. ; Lee, M. H. ; Kim, J. W. ; Kim, J. H. ; Shin, Y. K. ; Rho, J. K. ; Kwack, K. B. ; Lee, Wang Jae ; Han, Bok Ghee. / CD19 signalling improves the Epstein-Barr virus-induced immortalization of human B cell. In: Cell Proliferation. 2005 ; Vol. 38, No. 1. pp. 35-45.
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abstract = "Epstein-Barr virus (EBV) infection in vitro immortalizes primary B cells and generates B lymphoblastoid cell lines (LCLs). These EBV-LCLs have been used for several purposes in immunological and genetic studies, but some trials involving these transformations fail for unknown reasons, and several EBV-LCLs do not grow in normal culture. In this study, we improved the immortalization method by CD19 and B-cell receptor (BCR) co-ligation. This method shortens the time required for the immortalization and generation of EBV-LCLs but does not alter the cell phenotype of the LCLs nor the expression of the EBV genes. In particular, the CD19 and BCR coligation method was found to be the most effective method examined. EBV-infected B cells induced by CD 9 and/or BCR ligation expressed the intracellular latent membrane protein LMP-1 earlier than EBV-infected B cells, and the expression of intracellular LMP-1 was found to be closely related to the time of immortalization. These results suggest that the modified method, using CD19 and/or BCR ligation, may efficiently generate EBV-LCLs, by expressing intracellular LMP-1 at an early stage.",
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Hur, DY, Lee, MH, Kim, JW, Kim, JH, Shin, YK, Rho, JK, Kwack, KB, Lee, WJ & Han, BG 2005, 'CD19 signalling improves the Epstein-Barr virus-induced immortalization of human B cell', Cell Proliferation, vol. 38, no. 1, pp. 35-45. https://doi.org/10.1111/j.1365-2184.2005.00328.x

CD19 signalling improves the Epstein-Barr virus-induced immortalization of human B cell. / Hur, D. Y.; Lee, M. H.; Kim, J. W.; Kim, J. H.; Shin, Y. K.; Rho, J. K.; Kwack, K. B.; Lee, Wang Jae; Han, Bok Ghee.

In: Cell Proliferation, Vol. 38, No. 1, 01.02.2005, p. 35-45.

Research output: Contribution to journalArticleResearchpeer-review

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AB - Epstein-Barr virus (EBV) infection in vitro immortalizes primary B cells and generates B lymphoblastoid cell lines (LCLs). These EBV-LCLs have been used for several purposes in immunological and genetic studies, but some trials involving these transformations fail for unknown reasons, and several EBV-LCLs do not grow in normal culture. In this study, we improved the immortalization method by CD19 and B-cell receptor (BCR) co-ligation. This method shortens the time required for the immortalization and generation of EBV-LCLs but does not alter the cell phenotype of the LCLs nor the expression of the EBV genes. In particular, the CD19 and BCR coligation method was found to be the most effective method examined. EBV-infected B cells induced by CD 9 and/or BCR ligation expressed the intracellular latent membrane protein LMP-1 earlier than EBV-infected B cells, and the expression of intracellular LMP-1 was found to be closely related to the time of immortalization. These results suggest that the modified method, using CD19 and/or BCR ligation, may efficiently generate EBV-LCLs, by expressing intracellular LMP-1 at an early stage.

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