Cation-selective electropreconcentration

Il Hyung Shin, Ki Jung Kim, Jiman Kim, Hee Chan Kim, Honggu Chun

Research output: Contribution to journalArticleResearchpeer-review

14 Citations (Scopus)

Abstract

A cation-selective microfluidic sample preconcentration system is described. The cation sample was electropreconcentrated using a reversed-direction electroosmotic flow (EOF) and an anion-permselective filter, where an electric double layer (EDL) overlap condition existed. The anion-permselective filter between microchannels was fabricated by three different methods: 1) extending a positively charged, nanoporous, polymer membrane by photopolymerization of poly(diallyldimethylammonium chloride) (PDADMAC); 2) etching a nanochannel and then coating it with a positively-charged monomer, N-[3-(trimethoxysilyl)propyl]-N′-(4- vinylbenzyl)ethylenediamine hydrochloride (TMSVE); and, 3) etching a nanochannel and then coating it with a positively-charged, pre-formed polymer, polyE-323. The EOF direction in the microchannel was reversed by both TMSVE and polyE-323 coatings. The cation-selective preconcentration was investigated using charged fluorescent dyes and tetramethylrhodamine isothiocyanate (TRITC)-tagged peptides/proteins. The preconcentration in the three different systems was compared with respect to efficiency, dependence on buffer concentration and pH, tolerable flow rate, and sample adsorption. Both TMSVE- and polyE-323-coated nanochannels showed robust preconcentration at high flow rates, whereas the PDADMAC membrane maintained anion-permselectivity at higher buffer concentrations. The TMSVE-coated nanochannels showed a more stable preconcentration process, whereas the polyE-323-coated nanochannels showed a lower peptide sample adsorption and robust efficiency under a wide range of buffer pHs. The system described here can potentially be used for the preconcentration of cationic peptides/proteins on microfluidic devices for subsequent analyses.

Original languageEnglish
Pages (from-to)1811-1815
Number of pages5
JournalLab on a Chip
Volume14
Issue number11
DOIs
StatePublished - 7 Jun 2014

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Electroosmosis
Peptides
Anions
Cations
Buffers
ethylenediamine
Negative ions
Positive ions
Lab-On-A-Chip Devices
Microchannels
Microfluidics
Coatings
Adsorption
Etching
Polymers
Flow rate
Proteins
Membranes
Photopolymerization
Fluorescent Dyes

Cite this

Shin, I. H., Kim, K. J., Kim, J., Kim, H. C., & Chun, H. (2014). Cation-selective electropreconcentration. Lab on a Chip, 14(11), 1811-1815. https://doi.org/10.1039/c4lc00024b
Shin, Il Hyung ; Kim, Ki Jung ; Kim, Jiman ; Kim, Hee Chan ; Chun, Honggu. / Cation-selective electropreconcentration. In: Lab on a Chip. 2014 ; Vol. 14, No. 11. pp. 1811-1815.
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Shin, IH, Kim, KJ, Kim, J, Kim, HC & Chun, H 2014, 'Cation-selective electropreconcentration', Lab on a Chip, vol. 14, no. 11, pp. 1811-1815. https://doi.org/10.1039/c4lc00024b

Cation-selective electropreconcentration. / Shin, Il Hyung; Kim, Ki Jung; Kim, Jiman; Kim, Hee Chan; Chun, Honggu.

In: Lab on a Chip, Vol. 14, No. 11, 07.06.2014, p. 1811-1815.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Cation-selective electropreconcentration

AU - Shin, Il Hyung

AU - Kim, Ki Jung

AU - Kim, Jiman

AU - Kim, Hee Chan

AU - Chun, Honggu

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Y1 - 2014/6/7

N2 - A cation-selective microfluidic sample preconcentration system is described. The cation sample was electropreconcentrated using a reversed-direction electroosmotic flow (EOF) and an anion-permselective filter, where an electric double layer (EDL) overlap condition existed. The anion-permselective filter between microchannels was fabricated by three different methods: 1) extending a positively charged, nanoporous, polymer membrane by photopolymerization of poly(diallyldimethylammonium chloride) (PDADMAC); 2) etching a nanochannel and then coating it with a positively-charged monomer, N-[3-(trimethoxysilyl)propyl]-N′-(4- vinylbenzyl)ethylenediamine hydrochloride (TMSVE); and, 3) etching a nanochannel and then coating it with a positively-charged, pre-formed polymer, polyE-323. The EOF direction in the microchannel was reversed by both TMSVE and polyE-323 coatings. The cation-selective preconcentration was investigated using charged fluorescent dyes and tetramethylrhodamine isothiocyanate (TRITC)-tagged peptides/proteins. The preconcentration in the three different systems was compared with respect to efficiency, dependence on buffer concentration and pH, tolerable flow rate, and sample adsorption. Both TMSVE- and polyE-323-coated nanochannels showed robust preconcentration at high flow rates, whereas the PDADMAC membrane maintained anion-permselectivity at higher buffer concentrations. The TMSVE-coated nanochannels showed a more stable preconcentration process, whereas the polyE-323-coated nanochannels showed a lower peptide sample adsorption and robust efficiency under a wide range of buffer pHs. The system described here can potentially be used for the preconcentration of cationic peptides/proteins on microfluidic devices for subsequent analyses.

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U2 - 10.1039/c4lc00024b

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Shin IH, Kim KJ, Kim J, Kim HC, Chun H. Cation-selective electropreconcentration. Lab on a Chip. 2014 Jun 7;14(11):1811-1815. https://doi.org/10.1039/c4lc00024b