C-terminal binding protein-mediated transcriptional repression is regulated by X-linked inhibitor of apoptosis protein

Ji Sun Lee, Sun Kyung Lee, Hong-Duk Youn, Soon Ji Yoo

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

Inhibitors of Apoptosis Proteins (IAPs) are known as the key negative regulators of apoptosis. To explore new functions of IAPs, we sought to identify proteins that interact with Diap1 in insect S2 cells. We found that Diap1 bound to Drosophila C-terminal binding protein (dCtBP), which is a transcriptional co-repressor. CtBP1 also interacted with X-linked inhibitor of apoptosis protein (XIAP) in human cells. CtBPs were ubiquitinated by IAPs and targeted for proteasome-mediated degradation. Finally, the expression of CtBP1 target genes was regulated by XIAP expression. This is the first report to demonstrate that XIAP specifically regulates CtBP1, suggesting that XIAP may play a role in regulating CtBP1-mediated transcriptional repression by regulating the level of CtBP1.

Original languageEnglish
Pages (from-to)175-181
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume417
Issue number1
DOIs
StatePublished - 6 Jan 2012

Fingerprint

X-Linked Inhibitor of Apoptosis Protein
Inhibitor of Apoptosis Proteins
Co-Repressor Proteins
Proteasome Endopeptidase Complex
Insects
Apoptosis
Genes
Cells
Degradation
C-terminal binding protein
Proteins

Keywords

  • Apoptosis
  • CtBP
  • Transcriptional repression
  • Ubiquitin proteasome system
  • XIAP

Cite this

@article{de2c95e4a73446e0b8ef00482be45c6c,
title = "C-terminal binding protein-mediated transcriptional repression is regulated by X-linked inhibitor of apoptosis protein",
abstract = "Inhibitors of Apoptosis Proteins (IAPs) are known as the key negative regulators of apoptosis. To explore new functions of IAPs, we sought to identify proteins that interact with Diap1 in insect S2 cells. We found that Diap1 bound to Drosophila C-terminal binding protein (dCtBP), which is a transcriptional co-repressor. CtBP1 also interacted with X-linked inhibitor of apoptosis protein (XIAP) in human cells. CtBPs were ubiquitinated by IAPs and targeted for proteasome-mediated degradation. Finally, the expression of CtBP1 target genes was regulated by XIAP expression. This is the first report to demonstrate that XIAP specifically regulates CtBP1, suggesting that XIAP may play a role in regulating CtBP1-mediated transcriptional repression by regulating the level of CtBP1.",
keywords = "Apoptosis, CtBP, Transcriptional repression, Ubiquitin proteasome system, XIAP",
author = "Lee, {Ji Sun} and Lee, {Sun Kyung} and Hong-Duk Youn and Yoo, {Soon Ji}",
year = "2012",
month = "1",
day = "6",
doi = "10.1016/j.bbrc.2011.11.080",
language = "English",
volume = "417",
pages = "175--181",
journal = "Biochemical and biophysical research communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

C-terminal binding protein-mediated transcriptional repression is regulated by X-linked inhibitor of apoptosis protein. / Lee, Ji Sun; Lee, Sun Kyung; Youn, Hong-Duk; Yoo, Soon Ji.

In: Biochemical and Biophysical Research Communications, Vol. 417, No. 1, 06.01.2012, p. 175-181.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - C-terminal binding protein-mediated transcriptional repression is regulated by X-linked inhibitor of apoptosis protein

AU - Lee, Ji Sun

AU - Lee, Sun Kyung

AU - Youn, Hong-Duk

AU - Yoo, Soon Ji

PY - 2012/1/6

Y1 - 2012/1/6

N2 - Inhibitors of Apoptosis Proteins (IAPs) are known as the key negative regulators of apoptosis. To explore new functions of IAPs, we sought to identify proteins that interact with Diap1 in insect S2 cells. We found that Diap1 bound to Drosophila C-terminal binding protein (dCtBP), which is a transcriptional co-repressor. CtBP1 also interacted with X-linked inhibitor of apoptosis protein (XIAP) in human cells. CtBPs were ubiquitinated by IAPs and targeted for proteasome-mediated degradation. Finally, the expression of CtBP1 target genes was regulated by XIAP expression. This is the first report to demonstrate that XIAP specifically regulates CtBP1, suggesting that XIAP may play a role in regulating CtBP1-mediated transcriptional repression by regulating the level of CtBP1.

AB - Inhibitors of Apoptosis Proteins (IAPs) are known as the key negative regulators of apoptosis. To explore new functions of IAPs, we sought to identify proteins that interact with Diap1 in insect S2 cells. We found that Diap1 bound to Drosophila C-terminal binding protein (dCtBP), which is a transcriptional co-repressor. CtBP1 also interacted with X-linked inhibitor of apoptosis protein (XIAP) in human cells. CtBPs were ubiquitinated by IAPs and targeted for proteasome-mediated degradation. Finally, the expression of CtBP1 target genes was regulated by XIAP expression. This is the first report to demonstrate that XIAP specifically regulates CtBP1, suggesting that XIAP may play a role in regulating CtBP1-mediated transcriptional repression by regulating the level of CtBP1.

KW - Apoptosis

KW - CtBP

KW - Transcriptional repression

KW - Ubiquitin proteasome system

KW - XIAP

UR - http://www.scopus.com/inward/record.url?scp=84855820506&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2011.11.080

DO - 10.1016/j.bbrc.2011.11.080

M3 - Article

VL - 417

SP - 175

EP - 181

JO - Biochemical and biophysical research communications

JF - Biochemical and biophysical research communications

SN - 0006-291X

IS - 1

ER -