B cell-specific expression of B7-2 is required for follicular Th cell function in response to vaccinia virus

Samira Salek-Ardakani, Youn Soo Choi, Mohammed Rafii El Idrissi Benhnia, Rachel Flynn, Ramon Arens, Stephen Shoenberger, Shane Crotty, Michael Croft, Shahram Salek-Ardakani

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Abstract

Follicular Th (TFH) cells are specialized in provision of help to B cells that is essential for promoting protective Ab responses. CD28/B7 (B7-1 and B7-2) interactions are required for germinal center (GC) formation, but it is not clear if they simply support activation of naive CD4 T cells during initiation of responses by dendritic cells or if they directly control T FH cells and/or directly influence follicular B cell differentiation. Using a model of vaccinia virus infection, we show that B7-2 but not B7-1 deficiency profoundly impaired T FH cell development but did not affect CD4 T cell priming and Th1 differentiation. Consistent with this, B7-2 but not B7-1 was required for acquisition of GC B cell phenotype, plasma cell generation, and virus-specific neutralizing Ab responses. Mixed adoptive transfer experiments indicated that bidirectional interactions between CD28 expressed on activated T cells and B7-2 expressed on follicular B cells were essential for maintenance of the T FH phenotype and GC B cell development. Our data provide new insight into the source and nature of molecules required for T FH cells to direct GC B cell responses.

Original languageEnglish
Pages (from-to)5294-5303
Number of pages10
JournalJournal of Immunology
Volume186
Issue number9
DOIs
StatePublished - 1 May 2011

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Vaccinia virus
B-Lymphocytes
Germinal Center
T-Lymphocytes
Phenotype
Adoptive Transfer
Virus Diseases
Plasma Cells
Dendritic Cells
Cell Differentiation
Maintenance
Viruses

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Salek-Ardakani, S., Choi, Y. S., Benhnia, M. R. E. I., Flynn, R., Arens, R., Shoenberger, S., ... Salek-Ardakani, S. (2011). B cell-specific expression of B7-2 is required for follicular Th cell function in response to vaccinia virus. Journal of Immunology, 186(9), 5294-5303. https://doi.org/10.4049/jimmunol.1100406
Salek-Ardakani, Samira ; Choi, Youn Soo ; Benhnia, Mohammed Rafii El Idrissi ; Flynn, Rachel ; Arens, Ramon ; Shoenberger, Stephen ; Crotty, Shane ; Croft, Michael ; Salek-Ardakani, Shahram. / B cell-specific expression of B7-2 is required for follicular Th cell function in response to vaccinia virus. In: Journal of Immunology. 2011 ; Vol. 186, No. 9. pp. 5294-5303.
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abstract = "Follicular Th (TFH) cells are specialized in provision of help to B cells that is essential for promoting protective Ab responses. CD28/B7 (B7-1 and B7-2) interactions are required for germinal center (GC) formation, but it is not clear if they simply support activation of naive CD4 T cells during initiation of responses by dendritic cells or if they directly control T FH cells and/or directly influence follicular B cell differentiation. Using a model of vaccinia virus infection, we show that B7-2 but not B7-1 deficiency profoundly impaired T FH cell development but did not affect CD4 T cell priming and Th1 differentiation. Consistent with this, B7-2 but not B7-1 was required for acquisition of GC B cell phenotype, plasma cell generation, and virus-specific neutralizing Ab responses. Mixed adoptive transfer experiments indicated that bidirectional interactions between CD28 expressed on activated T cells and B7-2 expressed on follicular B cells were essential for maintenance of the T FH phenotype and GC B cell development. Our data provide new insight into the source and nature of molecules required for T FH cells to direct GC B cell responses.",
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Salek-Ardakani, S, Choi, YS, Benhnia, MREI, Flynn, R, Arens, R, Shoenberger, S, Crotty, S, Croft, M & Salek-Ardakani, S 2011, 'B cell-specific expression of B7-2 is required for follicular Th cell function in response to vaccinia virus', Journal of Immunology, vol. 186, no. 9, pp. 5294-5303. https://doi.org/10.4049/jimmunol.1100406

B cell-specific expression of B7-2 is required for follicular Th cell function in response to vaccinia virus. / Salek-Ardakani, Samira; Choi, Youn Soo; Benhnia, Mohammed Rafii El Idrissi; Flynn, Rachel; Arens, Ramon; Shoenberger, Stephen; Crotty, Shane; Croft, Michael; Salek-Ardakani, Shahram.

In: Journal of Immunology, Vol. 186, No. 9, 01.05.2011, p. 5294-5303.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Benhnia, Mohammed Rafii El Idrissi

AU - Flynn, Rachel

AU - Arens, Ramon

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AU - Croft, Michael

AU - Salek-Ardakani, Shahram

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