B-1 cells in the bone marrow are a significant source of natural IgM

Youn Soo Choi, Jacquelyn A. Dieter, Kristina Rothaeusler, Zheng Luo, Nicole Baumgarth

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Abstract

Natural IgM antibodies secreted in the absence of antigenic challenge are important contributors to antimicrobial immunity and tissue homeostasis. Early studies identified BM and, to a lesser extent the spleen, as main tissue sources of this spontaneously secreted IgM. However, the responsible B-cell subset has never been identified. Using multicolor flow cytometry, cell sorting and chimeric mice in which B-1 and B-2 cells and their secreted antibodies are distinguished by their Ig-allotype, we unequivocally identify the natural IgM-secreting cells in spleen and, for the first time, in the BM as IgM + IgD lo/-CD19 hi CD43 + CD5 +/- B-1 cells. The newly identified population of BM B-1 cells shows many of the phenotypic characteristics of splenic B-1 cells but is distinct from B-1 cells in the peritoneal cavity, which generate at best very small amounts of IgM. Antibody-secreting spleen and BM B-1 cells are distinct also from terminally differentiated plasma cells generated from antigen-induced conventional B cells, as they express high levels of surface IgM and CD19 and lack expression of CD138. Overall, these data identify populations of non-terminally differentiated B-1 cells in spleen and BM as the most significant producers of natural IgM.

Original languageEnglish
Pages (from-to)120-129
Number of pages10
JournalEuropean Journal of Immunology
Volume42
Issue number1
DOIs
StatePublished - 1 Jan 2012

Fingerprint

Bone Marrow Cells
Immunoglobulin M
Spleen
Antibodies
Immunoglobulin Allotypes
B-Lymphocyte Subsets
Immunoglobulin D
Peritoneal Cavity
Plasma Cells
Population
Immunity
Flow Cytometry
Homeostasis
B-Lymphocytes
Antigens

Keywords

  • Animal model
  • Antibodies
  • B cells
  • BM
  • Immunoglobulins

Cite this

Choi, Youn Soo ; Dieter, Jacquelyn A. ; Rothaeusler, Kristina ; Luo, Zheng ; Baumgarth, Nicole. / B-1 cells in the bone marrow are a significant source of natural IgM. In: European Journal of Immunology. 2012 ; Vol. 42, No. 1. pp. 120-129.
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B-1 cells in the bone marrow are a significant source of natural IgM. / Choi, Youn Soo; Dieter, Jacquelyn A.; Rothaeusler, Kristina; Luo, Zheng; Baumgarth, Nicole.

In: European Journal of Immunology, Vol. 42, No. 1, 01.01.2012, p. 120-129.

Research output: Contribution to journalArticleResearchpeer-review

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