Augmented osteogenesis of mesenchymal stem cells using a fragmented Runx2 mixed with cell-penetrating, dimeric a-helical peptide

So Hee Nam, Yan Lee, Joon Hyung Ahn, Chun Kee Chung, Hee Jin Yang, Sung Bae Park, Sangmok Jang

Research output: Contribution to journalArticle

Abstract

The intracellular delivery of transcription factor/cofactor using cell penetrating peptide (CPP) can lead to selective osteogenesis. The present work investigates the cell-penetrating potential of the a cyclic, α‐helical cell-penetrating peptide based on leucine and lysine residues (cLK) for intracellular delivery in MC3T3 cells and the osteogenic effects of a C-terminal proline‑serine‑threonine-rich (PST) domain of Runx2 using cLK in rat mesenchymal stem cells (MSCs). We confirmed that the combination of cLK and fluorescein 5-isothiocyanate (FITC)-fragmented-Runx2 (fRunx2) showed an enhanced cell-penetrating activity of FITC-fRunx2 compared with FITC-fRunx2 alone. In addition, the fRunx2-cLK group showed strong staining with alizarin red compared with other groups and the degree of alizarin red staining in the fRunx2-cLK group was also 1.2-fold higher than that in the fRunx2-Tat group. The ALP and osteocalcin gene expression levels in the fRunx2-cLK group were higher than those in the other groups. The fRunx2 transferred effectively into the cytoplasm aided by the cLK peptide and augmented the osteogenic differentiation of MSCs.

Original languageEnglish
Article number105210
JournalEuropean Journal of Pharmaceutical Sciences
Volume144
DOIs
StatePublished - 1 Mar 2020

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Keywords

  • Cell penetrating peptide
  • Mesenchymal stem cell
  • Osteoblast
  • Osteogenesis
  • Runx2
  • Spine

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