Augmentation cystoplasty using hydroxapatite/chitosan composite sheet seeded with autologous muscle-derived stem cells

Seok Soo Byun, Yong Sik Chung, Sang Sub Lee, Haet Nim Lee, Ji Young Lee, Ji Youl Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose: This study was designed to investigate the feasibility of a hydroxyapatite/chitosan (HAp/chitosan) composite, seeded with autologous muscle-derived stem cells, as a partial bladder substitute in rats. Materials and Methods: Muscle-derived stem cells were isolated from the gastrocnemius muscle of 6 female Sprague-Dawley rats, using the preplate technique, and cultured on HAp/chitosan composite sheets. Sheets with 10mm diameters were implanted into the urinary bladder of rats following a hemicystectomy in an autologous fashion. Three rats were sacrificed 4 and 8 weeks postoperatively, and the morphological changes subsequently assessed by H&E and immunofluorescence staining using DAPI, myogenin and α-smooth muscle actin (SMA). Results: All rats survived the scheduled duration. Adequate epithelialization was observed to be completed after postoperative week 4. Abundant muscle bundles, showing positive α-SMA staining, were observed after the 4th week. The bladder shape was well preserved after the 8th week. Ingrowing smooth muscles were observed on the periphery of the composite and muscular bundles, with positive myogenin immunostaining in the middle of the composite. Conclusions: A HAp/chitosan composite sheet, seeded with autologous muscle-derived stem cells, showed a degree of skeletal muscle differentiation 8 weeks after augmentation cystoplasty, in an autologous fashion. This new material seeded with muscle-derived stem cells may, in the future, prove to be a viable option as a partial bladder substitute.

Original languageEnglish
Pages (from-to)433-438
Number of pages6
JournalKorean Journal of Urology
Volume48
Issue number4
DOIs
StatePublished - Apr 2007

Fingerprint

Chitosan
Stem Cells
Urinary Bladder
Muscles
Durapatite
Myogenin
Smooth Muscle
Actins
Skeletal Muscle
Staining and Labeling
Fluorescent Antibody Technique
Sprague Dawley Rats

Keywords

  • Atrophy
  • Bladder
  • Regeneration
  • Stem cells

Cite this

Byun, Seok Soo ; Chung, Yong Sik ; Lee, Sang Sub ; Lee, Haet Nim ; Lee, Ji Young ; Lee, Ji Youl. / Augmentation cystoplasty using hydroxapatite/chitosan composite sheet seeded with autologous muscle-derived stem cells. In: Korean Journal of Urology. 2007 ; Vol. 48, No. 4. pp. 433-438.
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abstract = "Purpose: This study was designed to investigate the feasibility of a hydroxyapatite/chitosan (HAp/chitosan) composite, seeded with autologous muscle-derived stem cells, as a partial bladder substitute in rats. Materials and Methods: Muscle-derived stem cells were isolated from the gastrocnemius muscle of 6 female Sprague-Dawley rats, using the preplate technique, and cultured on HAp/chitosan composite sheets. Sheets with 10mm diameters were implanted into the urinary bladder of rats following a hemicystectomy in an autologous fashion. Three rats were sacrificed 4 and 8 weeks postoperatively, and the morphological changes subsequently assessed by H&E and immunofluorescence staining using DAPI, myogenin and α-smooth muscle actin (SMA). Results: All rats survived the scheduled duration. Adequate epithelialization was observed to be completed after postoperative week 4. Abundant muscle bundles, showing positive α-SMA staining, were observed after the 4th week. The bladder shape was well preserved after the 8th week. Ingrowing smooth muscles were observed on the periphery of the composite and muscular bundles, with positive myogenin immunostaining in the middle of the composite. Conclusions: A HAp/chitosan composite sheet, seeded with autologous muscle-derived stem cells, showed a degree of skeletal muscle differentiation 8 weeks after augmentation cystoplasty, in an autologous fashion. This new material seeded with muscle-derived stem cells may, in the future, prove to be a viable option as a partial bladder substitute.",
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Augmentation cystoplasty using hydroxapatite/chitosan composite sheet seeded with autologous muscle-derived stem cells. / Byun, Seok Soo; Chung, Yong Sik; Lee, Sang Sub; Lee, Haet Nim; Lee, Ji Young; Lee, Ji Youl.

In: Korean Journal of Urology, Vol. 48, No. 4, 04.2007, p. 433-438.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Augmentation cystoplasty using hydroxapatite/chitosan composite sheet seeded with autologous muscle-derived stem cells

AU - Byun, Seok Soo

AU - Chung, Yong Sik

AU - Lee, Sang Sub

AU - Lee, Haet Nim

AU - Lee, Ji Young

AU - Lee, Ji Youl

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N2 - Purpose: This study was designed to investigate the feasibility of a hydroxyapatite/chitosan (HAp/chitosan) composite, seeded with autologous muscle-derived stem cells, as a partial bladder substitute in rats. Materials and Methods: Muscle-derived stem cells were isolated from the gastrocnemius muscle of 6 female Sprague-Dawley rats, using the preplate technique, and cultured on HAp/chitosan composite sheets. Sheets with 10mm diameters were implanted into the urinary bladder of rats following a hemicystectomy in an autologous fashion. Three rats were sacrificed 4 and 8 weeks postoperatively, and the morphological changes subsequently assessed by H&E and immunofluorescence staining using DAPI, myogenin and α-smooth muscle actin (SMA). Results: All rats survived the scheduled duration. Adequate epithelialization was observed to be completed after postoperative week 4. Abundant muscle bundles, showing positive α-SMA staining, were observed after the 4th week. The bladder shape was well preserved after the 8th week. Ingrowing smooth muscles were observed on the periphery of the composite and muscular bundles, with positive myogenin immunostaining in the middle of the composite. Conclusions: A HAp/chitosan composite sheet, seeded with autologous muscle-derived stem cells, showed a degree of skeletal muscle differentiation 8 weeks after augmentation cystoplasty, in an autologous fashion. This new material seeded with muscle-derived stem cells may, in the future, prove to be a viable option as a partial bladder substitute.

AB - Purpose: This study was designed to investigate the feasibility of a hydroxyapatite/chitosan (HAp/chitosan) composite, seeded with autologous muscle-derived stem cells, as a partial bladder substitute in rats. Materials and Methods: Muscle-derived stem cells were isolated from the gastrocnemius muscle of 6 female Sprague-Dawley rats, using the preplate technique, and cultured on HAp/chitosan composite sheets. Sheets with 10mm diameters were implanted into the urinary bladder of rats following a hemicystectomy in an autologous fashion. Three rats were sacrificed 4 and 8 weeks postoperatively, and the morphological changes subsequently assessed by H&E and immunofluorescence staining using DAPI, myogenin and α-smooth muscle actin (SMA). Results: All rats survived the scheduled duration. Adequate epithelialization was observed to be completed after postoperative week 4. Abundant muscle bundles, showing positive α-SMA staining, were observed after the 4th week. The bladder shape was well preserved after the 8th week. Ingrowing smooth muscles were observed on the periphery of the composite and muscular bundles, with positive myogenin immunostaining in the middle of the composite. Conclusions: A HAp/chitosan composite sheet, seeded with autologous muscle-derived stem cells, showed a degree of skeletal muscle differentiation 8 weeks after augmentation cystoplasty, in an autologous fashion. This new material seeded with muscle-derived stem cells may, in the future, prove to be a viable option as a partial bladder substitute.

KW - Atrophy

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