Atrial natriuretic peptide and c-type natriuretic peptide in spontaneously hypertensive rats and their vasorelaxing actions in vitro

Chi Ming Wei, Cheolho Kim, Ali A. Khraibi, Virginia M. Miller, John C. Burnett

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31 Citations (Scopus)

Abstract

The present study determined circulating concentrations of atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP) in Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR) and also investigated the vasorelaxing action of ANP and CNP on isolated contracted aorta. We also defined the vasorelaxing action of a novel and newly synthesized 27-amino acid chimera of ANP and CNP termed vasonatrin peptide (VNP), which we compared with ANP and CNP in WKY rats and SHR. Plasma and urinary cyclic GMP and sodium excretion were also investigated. Plasma ANP was increased in SHR in contrast to no change in circulating CNP. Plasma and urinary cyclic GMP and sodium excretion were no different between WKY rats and SHR. In WKY rats maximal relaxations to VNP in aortic rings without endothelium were greater than those to ANP and CNP. In SHR aortic rings the potency of VNP relaxation was preserved, the actions of ANP were enhanced, and the actions of CNP were markedly impaired. In association with these vasorelaxing actions, these data suggest that (1) circulating CNP is not different in SHR and WKY rats, but the aortic relaxing action of CNP is markedly impaired in SHR; (2) endogenous plasma ANP is significantly increased in SHR without associated increases in plasma or urinary cyclic GMP; (3) there is an increase in aortic relaxation to exogenous ANP in SHR; and (4) VNP has a potent endothelium-independent aortic relaxing action in both WKY rats and SHR. These data suggest differential regulation of ANP and CNP and their vascular actions in SHR. These data also suggest that VNP could have an important therapeutic role in hypertension.

Original languageEnglish
Pages (from-to)903-907
Number of pages5
JournalHypertension
Volume23
Issue number6
DOIs
StatePublished - 1 Jan 1994

Fingerprint

C-Type Natriuretic Peptide
Natriuretic Peptides
Atrial Natriuretic Factor
Inbred SHR Rats
Inbred WKY Rats
Cyclic GMP
Endothelium
In Vitro Techniques
Sodium
Blood Vessels
Aorta
polypeptide C
eel ventricular natriuretic peptide

Keywords

  • Antihypertensive agents
  • Atrial natriuretic peptide
  • Inbred SHR
  • Muscle smooth vascular
  • Peptides
  • Rats Inbred WKY rats

Cite this

Wei, Chi Ming ; Kim, Cheolho ; Khraibi, Ali A. ; Miller, Virginia M. ; Burnett, John C. / Atrial natriuretic peptide and c-type natriuretic peptide in spontaneously hypertensive rats and their vasorelaxing actions in vitro. In: Hypertension. 1994 ; Vol. 23, No. 6. pp. 903-907.
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abstract = "The present study determined circulating concentrations of atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP) in Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR) and also investigated the vasorelaxing action of ANP and CNP on isolated contracted aorta. We also defined the vasorelaxing action of a novel and newly synthesized 27-amino acid chimera of ANP and CNP termed vasonatrin peptide (VNP), which we compared with ANP and CNP in WKY rats and SHR. Plasma and urinary cyclic GMP and sodium excretion were also investigated. Plasma ANP was increased in SHR in contrast to no change in circulating CNP. Plasma and urinary cyclic GMP and sodium excretion were no different between WKY rats and SHR. In WKY rats maximal relaxations to VNP in aortic rings without endothelium were greater than those to ANP and CNP. In SHR aortic rings the potency of VNP relaxation was preserved, the actions of ANP were enhanced, and the actions of CNP were markedly impaired. In association with these vasorelaxing actions, these data suggest that (1) circulating CNP is not different in SHR and WKY rats, but the aortic relaxing action of CNP is markedly impaired in SHR; (2) endogenous plasma ANP is significantly increased in SHR without associated increases in plasma or urinary cyclic GMP; (3) there is an increase in aortic relaxation to exogenous ANP in SHR; and (4) VNP has a potent endothelium-independent aortic relaxing action in both WKY rats and SHR. These data suggest differential regulation of ANP and CNP and their vascular actions in SHR. These data also suggest that VNP could have an important therapeutic role in hypertension.",
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Atrial natriuretic peptide and c-type natriuretic peptide in spontaneously hypertensive rats and their vasorelaxing actions in vitro. / Wei, Chi Ming; Kim, Cheolho; Khraibi, Ali A.; Miller, Virginia M.; Burnett, John C.

In: Hypertension, Vol. 23, No. 6, 01.01.1994, p. 903-907.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Atrial natriuretic peptide and c-type natriuretic peptide in spontaneously hypertensive rats and their vasorelaxing actions in vitro

AU - Wei, Chi Ming

AU - Kim, Cheolho

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AU - Miller, Virginia M.

AU - Burnett, John C.

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AB - The present study determined circulating concentrations of atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP) in Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR) and also investigated the vasorelaxing action of ANP and CNP on isolated contracted aorta. We also defined the vasorelaxing action of a novel and newly synthesized 27-amino acid chimera of ANP and CNP termed vasonatrin peptide (VNP), which we compared with ANP and CNP in WKY rats and SHR. Plasma and urinary cyclic GMP and sodium excretion were also investigated. Plasma ANP was increased in SHR in contrast to no change in circulating CNP. Plasma and urinary cyclic GMP and sodium excretion were no different between WKY rats and SHR. In WKY rats maximal relaxations to VNP in aortic rings without endothelium were greater than those to ANP and CNP. In SHR aortic rings the potency of VNP relaxation was preserved, the actions of ANP were enhanced, and the actions of CNP were markedly impaired. In association with these vasorelaxing actions, these data suggest that (1) circulating CNP is not different in SHR and WKY rats, but the aortic relaxing action of CNP is markedly impaired in SHR; (2) endogenous plasma ANP is significantly increased in SHR without associated increases in plasma or urinary cyclic GMP; (3) there is an increase in aortic relaxation to exogenous ANP in SHR; and (4) VNP has a potent endothelium-independent aortic relaxing action in both WKY rats and SHR. These data suggest differential regulation of ANP and CNP and their vascular actions in SHR. These data also suggest that VNP could have an important therapeutic role in hypertension.

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KW - Muscle smooth vascular

KW - Peptides

KW - Rats Inbred WKY rats

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