ATP-sensitive K + channels composed of Kir6.1 and SUR2B subunits in guinea pig gastric myocytes

J. A.E. Hoon Sim, Dong K.I. Yang, K. I.M. Young Chul, J. I.N. Sung Park, Tong Mook Kang, Insuk So, K. I. Whan Kim

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25 Citations (Scopus)

Abstract

This study was designed to identify the single-channel properties and molecular entity of ATP-sensitive K + (K ATP ) channels, in guinea pig gastric myocytes with patchclamp recording and RT-PCR. Pinacidil and diazoxide activated KATP currents in a glibenclamide-sensitive manner. The open probability of channels was enhanced by the application of 10 μM pinacidil from 0.085 ± 0.04 to 0.20 ± 0.05 (n = 7) and was completely blocked by 10 μM glibenclamide. Single-channel conductance was 37.3 ± 2.5 pS (n = 4) between -80 and -20 mV in symmetrical K + gradient conditions. In inside-out mode, KATP channels showed no spontaneous openings and were activated by the application of nucleotide diphosphates to the cytoplasmic side. These single-channel properties are similar to those of the nucleotide diphosphate-dependent K + channels in vascular smooth muscle, which are composed of Kir6.1 and sulfonylurea receptor (SUR)2B. RT-PCR demonstrated the presence of Kir6.1, Kir6.2, and SUR2B in guinea pig stomach smooth muscle cells. These results suggest that K ATP channels in smooth muscle cells of the guinea pig stomach are composed of Kir6.1 and SUR2B.

Original languageEnglish
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume282
Issue number1 45-1
StatePublished - 27 Jun 2002

Fingerprint

Pinacidil
Muscle Cells
Stomach
Guinea Pigs
Glyburide
Diphosphates
Adenosine Triphosphate
Smooth Muscle Myocytes
Nucleotides
Sulfonylurea Receptors
Diazoxide
KATP Channels
Polymerase Chain Reaction
Vascular Smooth Muscle
uK-ATP-1 potassium channel

Keywords

  • Adenosine 5′-triphosphate-sensitive potassium channels
  • Guinea pig stomach
  • Kir6.1-sulfonurea receptor 2B
  • Smooth muscle cells

Cite this

Hoon Sim, J. A. E., Yang, D. K. I., Young Chul, K. I. M., Sung Park, J. I. N., Kang, T. M., So, I., & Whan Kim, K. I. (2002). ATP-sensitive K + channels composed of Kir6.1 and SUR2B subunits in guinea pig gastric myocytes American Journal of Physiology - Gastrointestinal and Liver Physiology, 282(1 45-1).
Hoon Sim, J. A.E. ; Yang, Dong K.I. ; Young Chul, K. I.M. ; Sung Park, J. I.N. ; Kang, Tong Mook ; So, Insuk ; Whan Kim, K. I. / ATP-sensitive K + channels composed of Kir6.1 and SUR2B subunits in guinea pig gastric myocytes In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2002 ; Vol. 282, No. 1 45-1.
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abstract = "This study was designed to identify the single-channel properties and molecular entity of ATP-sensitive K + (K ATP ) channels, in guinea pig gastric myocytes with patchclamp recording and RT-PCR. Pinacidil and diazoxide activated KATP currents in a glibenclamide-sensitive manner. The open probability of channels was enhanced by the application of 10 μM pinacidil from 0.085 ± 0.04 to 0.20 ± 0.05 (n = 7) and was completely blocked by 10 μM glibenclamide. Single-channel conductance was 37.3 ± 2.5 pS (n = 4) between -80 and -20 mV in symmetrical K + gradient conditions. In inside-out mode, KATP channels showed no spontaneous openings and were activated by the application of nucleotide diphosphates to the cytoplasmic side. These single-channel properties are similar to those of the nucleotide diphosphate-dependent K + channels in vascular smooth muscle, which are composed of Kir6.1 and sulfonylurea receptor (SUR)2B. RT-PCR demonstrated the presence of Kir6.1, Kir6.2, and SUR2B in guinea pig stomach smooth muscle cells. These results suggest that K ATP channels in smooth muscle cells of the guinea pig stomach are composed of Kir6.1 and SUR2B.",
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Hoon Sim, JAE, Yang, DKI, Young Chul, KIM, Sung Park, JIN, Kang, TM, So, I & Whan Kim, KI 2002, ' ATP-sensitive K + channels composed of Kir6.1 and SUR2B subunits in guinea pig gastric myocytes ', American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 282, no. 1 45-1.

ATP-sensitive K + channels composed of Kir6.1 and SUR2B subunits in guinea pig gastric myocytes . / Hoon Sim, J. A.E.; Yang, Dong K.I.; Young Chul, K. I.M.; Sung Park, J. I.N.; Kang, Tong Mook; So, Insuk; Whan Kim, K. I.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 282, No. 1 45-1, 27.06.2002.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - ATP-sensitive K + channels composed of Kir6.1 and SUR2B subunits in guinea pig gastric myocytes

AU - Hoon Sim, J. A.E.

AU - Yang, Dong K.I.

AU - Young Chul, K. I.M.

AU - Sung Park, J. I.N.

AU - Kang, Tong Mook

AU - So, Insuk

AU - Whan Kim, K. I.

PY - 2002/6/27

Y1 - 2002/6/27

N2 - This study was designed to identify the single-channel properties and molecular entity of ATP-sensitive K + (K ATP ) channels, in guinea pig gastric myocytes with patchclamp recording and RT-PCR. Pinacidil and diazoxide activated KATP currents in a glibenclamide-sensitive manner. The open probability of channels was enhanced by the application of 10 μM pinacidil from 0.085 ± 0.04 to 0.20 ± 0.05 (n = 7) and was completely blocked by 10 μM glibenclamide. Single-channel conductance was 37.3 ± 2.5 pS (n = 4) between -80 and -20 mV in symmetrical K + gradient conditions. In inside-out mode, KATP channels showed no spontaneous openings and were activated by the application of nucleotide diphosphates to the cytoplasmic side. These single-channel properties are similar to those of the nucleotide diphosphate-dependent K + channels in vascular smooth muscle, which are composed of Kir6.1 and sulfonylurea receptor (SUR)2B. RT-PCR demonstrated the presence of Kir6.1, Kir6.2, and SUR2B in guinea pig stomach smooth muscle cells. These results suggest that K ATP channels in smooth muscle cells of the guinea pig stomach are composed of Kir6.1 and SUR2B.

AB - This study was designed to identify the single-channel properties and molecular entity of ATP-sensitive K + (K ATP ) channels, in guinea pig gastric myocytes with patchclamp recording and RT-PCR. Pinacidil and diazoxide activated KATP currents in a glibenclamide-sensitive manner. The open probability of channels was enhanced by the application of 10 μM pinacidil from 0.085 ± 0.04 to 0.20 ± 0.05 (n = 7) and was completely blocked by 10 μM glibenclamide. Single-channel conductance was 37.3 ± 2.5 pS (n = 4) between -80 and -20 mV in symmetrical K + gradient conditions. In inside-out mode, KATP channels showed no spontaneous openings and were activated by the application of nucleotide diphosphates to the cytoplasmic side. These single-channel properties are similar to those of the nucleotide diphosphate-dependent K + channels in vascular smooth muscle, which are composed of Kir6.1 and sulfonylurea receptor (SUR)2B. RT-PCR demonstrated the presence of Kir6.1, Kir6.2, and SUR2B in guinea pig stomach smooth muscle cells. These results suggest that K ATP channels in smooth muscle cells of the guinea pig stomach are composed of Kir6.1 and SUR2B.

KW - Adenosine 5′-triphosphate-sensitive potassium channels

KW - Guinea pig stomach

KW - Kir6.1-sulfonurea receptor 2B

KW - Smooth muscle cells

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M3 - Article

VL - 282

JO - American Journal of Physiology - Gastrointestinal and Liver Physiology

JF - American Journal of Physiology - Gastrointestinal and Liver Physiology

SN - 0193-1857

IS - 1 45-1

ER -