Application of fluorescence in situ hybridization on cerebrospinal fluid cytospins for the detection of residual leukemic cells in patients with childhood acute lymphoblastic leukemia

Sang Mee Hwang, Hee Sue Park, Seungman Park, Sung Min Kim, Kyung Taek Hong, Yoon Hwan Chang, Dong Soon Lee

Research output: Contribution to journalArticle

Abstract

Objectives: Diagnosis of central nervous system involvement in acute lymphoblastic leukemia (ALL) requires morphologic expertise; therefore, we evaluated interphase fluorescence in situ hybridization (iFISH) of cerebrospinal fluid (CSF) cytospin preparations as a potential complementary test. Methods: Twenty-three CSF cytospin specimens from 13 pediatric patients with ALL were included. iFISH probes detecting BCR-ABL1, ETV6-RUNX1, and KMT2A rearrangement and CDKN2A deletion, which were present at initial diagnosis, were used on follow-up CSF cytospin specimens and were compared with cytology. Results: Seventeen (73.9%) follow-up specimens showed concordant results between iFISH and cytology. Two (8.7%) samples with discordant results were positive by iFISH but not by cytology; one (4.3%) was positive only by cytology. In the remaining three (13.0%) specimens, too few cells were available for cytology, whereas iFISH interpretation was possible. Conclusions: iFISH of CSF cytospin preparations improves malignant cell detection in pediatric ALL.

Original languageEnglish
Pages (from-to)416-423
Number of pages8
JournalAmerican Journal of Clinical Pathology
Volume151
Issue number4
DOIs
StatePublished - 1 Apr 2019

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Interphase
Fluorescence In Situ Hybridization
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Cerebrospinal Fluid
Cell Biology
Pediatrics
Central Nervous System

Keywords

  • Acute lymphoblastic leukemia
  • Cerebrospinal fluid
  • Cytospin
  • Fluorescence in situ hybridization

Cite this

@article{7b7d01be77bd42c28add7d611d917a79,
title = "Application of fluorescence in situ hybridization on cerebrospinal fluid cytospins for the detection of residual leukemic cells in patients with childhood acute lymphoblastic leukemia",
abstract = "Objectives: Diagnosis of central nervous system involvement in acute lymphoblastic leukemia (ALL) requires morphologic expertise; therefore, we evaluated interphase fluorescence in situ hybridization (iFISH) of cerebrospinal fluid (CSF) cytospin preparations as a potential complementary test. Methods: Twenty-three CSF cytospin specimens from 13 pediatric patients with ALL were included. iFISH probes detecting BCR-ABL1, ETV6-RUNX1, and KMT2A rearrangement and CDKN2A deletion, which were present at initial diagnosis, were used on follow-up CSF cytospin specimens and were compared with cytology. Results: Seventeen (73.9{\%}) follow-up specimens showed concordant results between iFISH and cytology. Two (8.7{\%}) samples with discordant results were positive by iFISH but not by cytology; one (4.3{\%}) was positive only by cytology. In the remaining three (13.0{\%}) specimens, too few cells were available for cytology, whereas iFISH interpretation was possible. Conclusions: iFISH of CSF cytospin preparations improves malignant cell detection in pediatric ALL.",
keywords = "Acute lymphoblastic leukemia, Cerebrospinal fluid, Cytospin, Fluorescence in situ hybridization",
author = "Hwang, {Sang Mee} and Park, {Hee Sue} and Seungman Park and Kim, {Sung Min} and Hong, {Kyung Taek} and Chang, {Yoon Hwan} and Lee, {Dong Soon}",
year = "2019",
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doi = "10.1093/AJCP/AQY160",
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pages = "416--423",
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T1 - Application of fluorescence in situ hybridization on cerebrospinal fluid cytospins for the detection of residual leukemic cells in patients with childhood acute lymphoblastic leukemia

AU - Hwang, Sang Mee

AU - Park, Hee Sue

AU - Park, Seungman

AU - Kim, Sung Min

AU - Hong, Kyung Taek

AU - Chang, Yoon Hwan

AU - Lee, Dong Soon

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Objectives: Diagnosis of central nervous system involvement in acute lymphoblastic leukemia (ALL) requires morphologic expertise; therefore, we evaluated interphase fluorescence in situ hybridization (iFISH) of cerebrospinal fluid (CSF) cytospin preparations as a potential complementary test. Methods: Twenty-three CSF cytospin specimens from 13 pediatric patients with ALL were included. iFISH probes detecting BCR-ABL1, ETV6-RUNX1, and KMT2A rearrangement and CDKN2A deletion, which were present at initial diagnosis, were used on follow-up CSF cytospin specimens and were compared with cytology. Results: Seventeen (73.9%) follow-up specimens showed concordant results between iFISH and cytology. Two (8.7%) samples with discordant results were positive by iFISH but not by cytology; one (4.3%) was positive only by cytology. In the remaining three (13.0%) specimens, too few cells were available for cytology, whereas iFISH interpretation was possible. Conclusions: iFISH of CSF cytospin preparations improves malignant cell detection in pediatric ALL.

AB - Objectives: Diagnosis of central nervous system involvement in acute lymphoblastic leukemia (ALL) requires morphologic expertise; therefore, we evaluated interphase fluorescence in situ hybridization (iFISH) of cerebrospinal fluid (CSF) cytospin preparations as a potential complementary test. Methods: Twenty-three CSF cytospin specimens from 13 pediatric patients with ALL were included. iFISH probes detecting BCR-ABL1, ETV6-RUNX1, and KMT2A rearrangement and CDKN2A deletion, which were present at initial diagnosis, were used on follow-up CSF cytospin specimens and were compared with cytology. Results: Seventeen (73.9%) follow-up specimens showed concordant results between iFISH and cytology. Two (8.7%) samples with discordant results were positive by iFISH but not by cytology; one (4.3%) was positive only by cytology. In the remaining three (13.0%) specimens, too few cells were available for cytology, whereas iFISH interpretation was possible. Conclusions: iFISH of CSF cytospin preparations improves malignant cell detection in pediatric ALL.

KW - Acute lymphoblastic leukemia

KW - Cerebrospinal fluid

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KW - Fluorescence in situ hybridization

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