Antioxidant effects of selenium on lung injury in paraquat intoxicated rats

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Context. Paraquat (PQ) causes lethal intoxication by inducing oxidant injury to the lung. Selenium is a cofactor for glutathione peroxidase (GPx), which is one of the major endogenous antioxidant enzymes. Objective. To determine whether selenium post-treatment activates GPx, decreases lung injury, and improves survival in PQ intoxicated rats. Materials and methods. Male SpraqueDawley rats were categorized into three groups: sham (n 6), PQ (n 12), and PQ Se (n 12). In the PQ and PQ Se groups, 50 mg/kg of PQ was administered intraperitoneally. After 10 minutes, 60 μg/kg of Se (PQ Se) or saline (PQ) was administered via the tail vein. Six rats per group were euthanized 6 hours or 24 hours later. Lung tissues were harvested for the measurement of GPx activity, reduced glutathione (GSH), glutathione disulfide (GSSG) and malondialdehyde (MDA) and for histological analysis. Using separated set of rats, survival of PQ (n 10) and PQ Se (n 10) were observed for 72 hours. Results. GPx activity in the PQ group at the 6-hour and 24-hour time points was lower than in the sham group (p <0.006). GPx activity in the PQ Se group at the 6-hour and 24-hour time points was higher than in the PQ group at the same time (p <0.006). GPx activity in the PQ Se group at 24 hours was higher than at 6-hour time point and also higher than in the sham group (p <0.006). The GSH/GSSG ratio in the PQ Se group at 24 hours was lower than that in the sham group (p <0.006). MDA levels in the PQ group at 6 hours and 24 hours were higher than in the sham group (p <0.006). MDA levels at 24 hours in the PQ Se group was lower than in the PQ group (p <0.006). Acute lung injury (ALI) scores in the PQ group at 6 hours and 24 hours were higher than in the sham group (p <0.006). ALI scores at 24 hours in the PQ Se group were lower than in the PQ group. Survival rates did not differ between PQ and PQ Se (p 0.869). Conclusion. Single dose of selenium post-treatment activates GPx and attenuates lipid peroxidation and lung injury early after paraquat intoxication, but does not improve 72 hours of survival.

Original languageEnglish
Pages (from-to)749-753
Number of pages5
JournalClinical Toxicology
Volume50
Issue number8
DOIs
StatePublished - 1 Sep 2012

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Paraquat
Lung Injury
Selenium
Rats
Antioxidants
Glutathione Peroxidase
Glutathione Disulfide
Malondialdehyde
Acute Lung Injury

Keywords

  • Antioxidants
  • Glutathione peroxidase
  • Lung injury
  • Paraquat
  • Selenium

Cite this

@article{0472eb56e19f476597350322be87b974,
title = "Antioxidant effects of selenium on lung injury in paraquat intoxicated rats",
abstract = "Context. Paraquat (PQ) causes lethal intoxication by inducing oxidant injury to the lung. Selenium is a cofactor for glutathione peroxidase (GPx), which is one of the major endogenous antioxidant enzymes. Objective. To determine whether selenium post-treatment activates GPx, decreases lung injury, and improves survival in PQ intoxicated rats. Materials and methods. Male SpraqueDawley rats were categorized into three groups: sham (n 6), PQ (n 12), and PQ Se (n 12). In the PQ and PQ Se groups, 50 mg/kg of PQ was administered intraperitoneally. After 10 minutes, 60 μg/kg of Se (PQ Se) or saline (PQ) was administered via the tail vein. Six rats per group were euthanized 6 hours or 24 hours later. Lung tissues were harvested for the measurement of GPx activity, reduced glutathione (GSH), glutathione disulfide (GSSG) and malondialdehyde (MDA) and for histological analysis. Using separated set of rats, survival of PQ (n 10) and PQ Se (n 10) were observed for 72 hours. Results. GPx activity in the PQ group at the 6-hour and 24-hour time points was lower than in the sham group (p <0.006). GPx activity in the PQ Se group at the 6-hour and 24-hour time points was higher than in the PQ group at the same time (p <0.006). GPx activity in the PQ Se group at 24 hours was higher than at 6-hour time point and also higher than in the sham group (p <0.006). The GSH/GSSG ratio in the PQ Se group at 24 hours was lower than that in the sham group (p <0.006). MDA levels in the PQ group at 6 hours and 24 hours were higher than in the sham group (p <0.006). MDA levels at 24 hours in the PQ Se group was lower than in the PQ group (p <0.006). Acute lung injury (ALI) scores in the PQ group at 6 hours and 24 hours were higher than in the sham group (p <0.006). ALI scores at 24 hours in the PQ Se group were lower than in the PQ group. Survival rates did not differ between PQ and PQ Se (p 0.869). Conclusion. Single dose of selenium post-treatment activates GPx and attenuates lipid peroxidation and lung injury early after paraquat intoxication, but does not improve 72 hours of survival.",
keywords = "Antioxidants, Glutathione peroxidase, Lung injury, Paraquat, Selenium",
author = "Kim, {Kyung Su} and Suh, {Gil Joon} and Kwon, {Woon Yong} and Kwak, {Young Ho} and Lee, {Kyoung Bun} and Lee, {Hui Jai} and Jeong, {Ki Young} and Lee, {Myung Woo}",
year = "2012",
month = "9",
day = "1",
doi = "10.3109/15563650.2012.708418",
language = "English",
volume = "50",
pages = "749--753",
journal = "Clinical Toxicology",
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}

Antioxidant effects of selenium on lung injury in paraquat intoxicated rats. / Kim, Kyung Su; Suh, Gil Joon; Kwon, Woon Yong; Kwak, Young Ho; Lee, Kyoung Bun; Lee, Hui Jai; Jeong, Ki Young; Lee, Myung Woo.

In: Clinical Toxicology, Vol. 50, No. 8, 01.09.2012, p. 749-753.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antioxidant effects of selenium on lung injury in paraquat intoxicated rats

AU - Kim, Kyung Su

AU - Suh, Gil Joon

AU - Kwon, Woon Yong

AU - Kwak, Young Ho

AU - Lee, Kyoung Bun

AU - Lee, Hui Jai

AU - Jeong, Ki Young

AU - Lee, Myung Woo

PY - 2012/9/1

Y1 - 2012/9/1

N2 - Context. Paraquat (PQ) causes lethal intoxication by inducing oxidant injury to the lung. Selenium is a cofactor for glutathione peroxidase (GPx), which is one of the major endogenous antioxidant enzymes. Objective. To determine whether selenium post-treatment activates GPx, decreases lung injury, and improves survival in PQ intoxicated rats. Materials and methods. Male SpraqueDawley rats were categorized into three groups: sham (n 6), PQ (n 12), and PQ Se (n 12). In the PQ and PQ Se groups, 50 mg/kg of PQ was administered intraperitoneally. After 10 minutes, 60 μg/kg of Se (PQ Se) or saline (PQ) was administered via the tail vein. Six rats per group were euthanized 6 hours or 24 hours later. Lung tissues were harvested for the measurement of GPx activity, reduced glutathione (GSH), glutathione disulfide (GSSG) and malondialdehyde (MDA) and for histological analysis. Using separated set of rats, survival of PQ (n 10) and PQ Se (n 10) were observed for 72 hours. Results. GPx activity in the PQ group at the 6-hour and 24-hour time points was lower than in the sham group (p <0.006). GPx activity in the PQ Se group at the 6-hour and 24-hour time points was higher than in the PQ group at the same time (p <0.006). GPx activity in the PQ Se group at 24 hours was higher than at 6-hour time point and also higher than in the sham group (p <0.006). The GSH/GSSG ratio in the PQ Se group at 24 hours was lower than that in the sham group (p <0.006). MDA levels in the PQ group at 6 hours and 24 hours were higher than in the sham group (p <0.006). MDA levels at 24 hours in the PQ Se group was lower than in the PQ group (p <0.006). Acute lung injury (ALI) scores in the PQ group at 6 hours and 24 hours were higher than in the sham group (p <0.006). ALI scores at 24 hours in the PQ Se group were lower than in the PQ group. Survival rates did not differ between PQ and PQ Se (p 0.869). Conclusion. Single dose of selenium post-treatment activates GPx and attenuates lipid peroxidation and lung injury early after paraquat intoxication, but does not improve 72 hours of survival.

AB - Context. Paraquat (PQ) causes lethal intoxication by inducing oxidant injury to the lung. Selenium is a cofactor for glutathione peroxidase (GPx), which is one of the major endogenous antioxidant enzymes. Objective. To determine whether selenium post-treatment activates GPx, decreases lung injury, and improves survival in PQ intoxicated rats. Materials and methods. Male SpraqueDawley rats were categorized into three groups: sham (n 6), PQ (n 12), and PQ Se (n 12). In the PQ and PQ Se groups, 50 mg/kg of PQ was administered intraperitoneally. After 10 minutes, 60 μg/kg of Se (PQ Se) or saline (PQ) was administered via the tail vein. Six rats per group were euthanized 6 hours or 24 hours later. Lung tissues were harvested for the measurement of GPx activity, reduced glutathione (GSH), glutathione disulfide (GSSG) and malondialdehyde (MDA) and for histological analysis. Using separated set of rats, survival of PQ (n 10) and PQ Se (n 10) were observed for 72 hours. Results. GPx activity in the PQ group at the 6-hour and 24-hour time points was lower than in the sham group (p <0.006). GPx activity in the PQ Se group at the 6-hour and 24-hour time points was higher than in the PQ group at the same time (p <0.006). GPx activity in the PQ Se group at 24 hours was higher than at 6-hour time point and also higher than in the sham group (p <0.006). The GSH/GSSG ratio in the PQ Se group at 24 hours was lower than that in the sham group (p <0.006). MDA levels in the PQ group at 6 hours and 24 hours were higher than in the sham group (p <0.006). MDA levels at 24 hours in the PQ Se group was lower than in the PQ group (p <0.006). Acute lung injury (ALI) scores in the PQ group at 6 hours and 24 hours were higher than in the sham group (p <0.006). ALI scores at 24 hours in the PQ Se group were lower than in the PQ group. Survival rates did not differ between PQ and PQ Se (p 0.869). Conclusion. Single dose of selenium post-treatment activates GPx and attenuates lipid peroxidation and lung injury early after paraquat intoxication, but does not improve 72 hours of survival.

KW - Antioxidants

KW - Glutathione peroxidase

KW - Lung injury

KW - Paraquat

KW - Selenium

UR - http://www.scopus.com/inward/record.url?scp=84865607744&partnerID=8YFLogxK

U2 - 10.3109/15563650.2012.708418

DO - 10.3109/15563650.2012.708418

M3 - Article

C2 - 22924652

AN - SCOPUS:84865607744

VL - 50

SP - 749

EP - 753

JO - Clinical Toxicology

JF - Clinical Toxicology

SN - 1556-3650

IS - 8

ER -