Anticancer effects of oligomeric proanthocyanidins on human colorectal cancer cell line, SNU-C4

Youn Jung Kim, Hae Jeong Park, Seo Hyun Yoon, Mi Ja Kim, Kang Hyun Leem, Joo Ho Chung, Hye Kyung Kim

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Aim: Oligomeric proanthocyanidins (OPC), natural polyphenolic compounds found in plants, are known to have antioxidant and anti-cancer effects. We investigated whether the anti-cancer effects of the OPC are induced by apoptosis on human colorectal cancer cell line, SNU-C4. Methods: Colorectal cancer cell line, SNU-C4 was cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum. The cytotoxic effect of OPC was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. To find out the apoptotic cell death, 4, 6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcription-polymerase chain reaction (RT-PCR), and caspase-3 enzyme assay were performed. Results: In this study, cytotoxic effect of OPC on SNU-C4 cells appeared in a dose-dependent manner. OPC treatment (100 μg/mL) revealed typical morphological apoptotic features. Additionally OPC treatment (100 μg/mL) increased level of BAX and CASPASE-3, and decreased level of BCL-2 mRNA expression. Caspase-3 enzyme activity was also significantly increased by treatment of OPC (100 μg/mL) compared with control. Conclusion: These data indicate that OPC caused cell death by apoptosis through caspase pathways on human colorectal cancer cell line, SNU-C4.

Original languageEnglish
Pages (from-to)4674-4678
Number of pages5
JournalWorld Journal of Gastroenterology
Volume11
Issue number30
DOIs
StatePublished - 14 Aug 2005

Fingerprint

Proanthocyanidins
Colorectal Neoplasms
Cell Line
Caspase 3
Cell Death
Apoptosis
DNA Nucleotidylexotransferase
Enzyme Assays
Caspases
Reverse Transcription
Neoplasms
Antioxidants
Staining and Labeling
Polymerase Chain Reaction
Messenger RNA
Enzymes

Keywords

  • Anticancer effects
  • Apoptosis
  • Colorectal cancer
  • Oligomeric pranthocyanidins

Cite this

Kim, Youn Jung ; Park, Hae Jeong ; Yoon, Seo Hyun ; Kim, Mi Ja ; Leem, Kang Hyun ; Chung, Joo Ho ; Kim, Hye Kyung. / Anticancer effects of oligomeric proanthocyanidins on human colorectal cancer cell line, SNU-C4. In: World Journal of Gastroenterology. 2005 ; Vol. 11, No. 30. pp. 4674-4678.
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abstract = "Aim: Oligomeric proanthocyanidins (OPC), natural polyphenolic compounds found in plants, are known to have antioxidant and anti-cancer effects. We investigated whether the anti-cancer effects of the OPC are induced by apoptosis on human colorectal cancer cell line, SNU-C4. Methods: Colorectal cancer cell line, SNU-C4 was cultured in RPMI 1640 medium supplemented with 10{\%} fetal bovine serum. The cytotoxic effect of OPC was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. To find out the apoptotic cell death, 4, 6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcription-polymerase chain reaction (RT-PCR), and caspase-3 enzyme assay were performed. Results: In this study, cytotoxic effect of OPC on SNU-C4 cells appeared in a dose-dependent manner. OPC treatment (100 μg/mL) revealed typical morphological apoptotic features. Additionally OPC treatment (100 μg/mL) increased level of BAX and CASPASE-3, and decreased level of BCL-2 mRNA expression. Caspase-3 enzyme activity was also significantly increased by treatment of OPC (100 μg/mL) compared with control. Conclusion: These data indicate that OPC caused cell death by apoptosis through caspase pathways on human colorectal cancer cell line, SNU-C4.",
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Anticancer effects of oligomeric proanthocyanidins on human colorectal cancer cell line, SNU-C4. / Kim, Youn Jung; Park, Hae Jeong; Yoon, Seo Hyun; Kim, Mi Ja; Leem, Kang Hyun; Chung, Joo Ho; Kim, Hye Kyung.

In: World Journal of Gastroenterology, Vol. 11, No. 30, 14.08.2005, p. 4674-4678.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Anticancer effects of oligomeric proanthocyanidins on human colorectal cancer cell line, SNU-C4

AU - Kim, Youn Jung

AU - Park, Hae Jeong

AU - Yoon, Seo Hyun

AU - Kim, Mi Ja

AU - Leem, Kang Hyun

AU - Chung, Joo Ho

AU - Kim, Hye Kyung

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N2 - Aim: Oligomeric proanthocyanidins (OPC), natural polyphenolic compounds found in plants, are known to have antioxidant and anti-cancer effects. We investigated whether the anti-cancer effects of the OPC are induced by apoptosis on human colorectal cancer cell line, SNU-C4. Methods: Colorectal cancer cell line, SNU-C4 was cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum. The cytotoxic effect of OPC was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. To find out the apoptotic cell death, 4, 6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcription-polymerase chain reaction (RT-PCR), and caspase-3 enzyme assay were performed. Results: In this study, cytotoxic effect of OPC on SNU-C4 cells appeared in a dose-dependent manner. OPC treatment (100 μg/mL) revealed typical morphological apoptotic features. Additionally OPC treatment (100 μg/mL) increased level of BAX and CASPASE-3, and decreased level of BCL-2 mRNA expression. Caspase-3 enzyme activity was also significantly increased by treatment of OPC (100 μg/mL) compared with control. Conclusion: These data indicate that OPC caused cell death by apoptosis through caspase pathways on human colorectal cancer cell line, SNU-C4.

AB - Aim: Oligomeric proanthocyanidins (OPC), natural polyphenolic compounds found in plants, are known to have antioxidant and anti-cancer effects. We investigated whether the anti-cancer effects of the OPC are induced by apoptosis on human colorectal cancer cell line, SNU-C4. Methods: Colorectal cancer cell line, SNU-C4 was cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum. The cytotoxic effect of OPC was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. To find out the apoptotic cell death, 4, 6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcription-polymerase chain reaction (RT-PCR), and caspase-3 enzyme assay were performed. Results: In this study, cytotoxic effect of OPC on SNU-C4 cells appeared in a dose-dependent manner. OPC treatment (100 μg/mL) revealed typical morphological apoptotic features. Additionally OPC treatment (100 μg/mL) increased level of BAX and CASPASE-3, and decreased level of BCL-2 mRNA expression. Caspase-3 enzyme activity was also significantly increased by treatment of OPC (100 μg/mL) compared with control. Conclusion: These data indicate that OPC caused cell death by apoptosis through caspase pathways on human colorectal cancer cell line, SNU-C4.

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