Anaerotignum faecicola sp. nov., isolated from human faeces

Seung Hyeon Choi, Ji Sun Kim, Jam Eon Park, Keun Chul Lee, Mi Kyung Eom, Byeong Seob Oh, Seung Yeob Yu, Se Won Kang, Kook Il Han, Min Kuk Suh, Dong Ho Lee, Hyuk Yoon, Byung Yong Kim, Je Hee Lee, Ju Huck Lee, Jung Sook Lee, Seung Hwan Park

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Abstract

A strictly anaerobic bacterium, designated as strain KGMB-03357T, was isolated from the faeces of a healthy Korean selected by Bundang Seoul National University based on health status. Cells of strain KGMB03357T are Gram-stain-positive, non-motile, non-spore-forming, and observed as straight or curved rods. The isolate grew at 10–45°C (optimum temperature of 40°C) and a pH range of 5.1–10.5 (optimum pH of 6.8). Analysis of phylogenetic trees based on the 16S rRNA gene sequences revealed that strain KGMB03357T forms a lineage within the genus Anaerotignum, and is most closely related to Anaerotignum lactatifermentans G17T (= KCTC 15066T, 96.1%), Anaerotignum propionicum DSM 1682T (= KCTC 5582T, 94.9%), Anaerotignum neopropionicum DSM 03847T (= KCTC 15564T, 94.9%), and Anaerotignum aminivorans SH021T (= KCTC 15705T, 94.8%). The ANI values between strain KGMB 03357T and members of the genus Anaerotignum were 73.3–71.0%, which are below the ANI criterion for interspecies identity. The DNA G + C content based on the whole-genome sequence is 47.3 mol%. The major cellular fatty acids of strain KGMB03357T are C16:0, C18:0, C18∶1 cis 9, and anteiso-C15∶0. Strain KGMB03357T contains meso-diaminopimelic acid as the diagnostic amino acid in the cell wall peptidoglycan. Based on the phenotypic, phylogenetic, and genomic properties, strain KGMB 03357T represents a novel species of the genus Anaerotignum, for which the name Anaerotignum faecicola sp. nov. is proposed. The type strain is KGMB03357T (= KCTC 15736T = DSM 107953T).

Original languageEnglish
Pages (from-to)1073-1078
Number of pages6
JournalJournal of Microbiology
Volume57
Issue number12
DOIs
StatePublished - 1 Dec 2019

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Keywords

  • Anaerotignum faecicola
  • human faeces
  • microbiota
  • taxonomy

Cite this

Choi, S. H., Kim, J. S., Park, J. E., Lee, K. C., Eom, M. K., Oh, B. S., Yu, S. Y., Kang, S. W., Han, K. I., Suh, M. K., Lee, D. H., Yoon, H., Kim, B. Y., Lee, J. H., Lee, J. H., Lee, J. S., & Park, S. H. (2019). Anaerotignum faecicola sp. nov., isolated from human faeces. Journal of Microbiology, 57(12), 1073-1078. https://doi.org/10.1007/s12275-019-9268-3