An RNA aptamer that specifically binds pancreatic adenocarcinoma up-regulated factor inhibits migration and growth of pancreatic cancer cells

Yun Hee Kim, Ho Jin Sung, Sukyoung Kim, Eun Ok Kim, Ji Won Lee, Ju Young Moon, Kyungho Choi, Ji Eun Jung, Yangsoon Lee, Sang Seok Koh, Sue Goo Rhee, Kyun Heo, In Hoo Kim

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Abstract

Previously, we reported that a novel secretory protein, pancreatic adenocarcinoma up-regulated factor (PAUF), which is highly expressed in pancreatic cancer and mediates the growth and metastasis of pancreatic cancer cells. In this study, we generated and characterized a 2'-fluoropyrimidine modified RNA aptamer (P12FR2) directed against human PAUF. P12FR2 binds specifically to human PAUF with an estimated apparent K D of 77. nM. P12FR2 aptamer inhibits PAUF-induced migration of PANC-1, human pancreatic cancer cells, in a wound healing assay. Moreover, intraperitoneal injection of P12FR2 decreased tumor growth by about 60% in an in vivo xenograft model with CFPAC-1 pancreatic cancer cells, without causing a loss of weight in the treated mice. Taken together, we propose here that PAUF-specific RNA aptamer, P12FR2, has the potential to be effective in the therapy of human pancreatic cancer.

Original languageEnglish
Pages (from-to)76-83
Number of pages8
JournalCancer Letters
Volume313
Issue number1
DOIs
StatePublished - 26 Dec 2011

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Nucleotide Aptamers
Pancreatic Neoplasms
Adenocarcinoma
Growth
Intraperitoneal Injections
Heterografts
Wound Healing
Weight Loss
Neoplasm Metastasis
Neoplasms
Proteins

Keywords

  • Anticancer drug
  • Aptamer
  • PAUF
  • Pancreatic cancer
  • SELEX

Cite this

Kim, Yun Hee ; Sung, Ho Jin ; Kim, Sukyoung ; Kim, Eun Ok ; Lee, Ji Won ; Moon, Ju Young ; Choi, Kyungho ; Jung, Ji Eun ; Lee, Yangsoon ; Koh, Sang Seok ; Rhee, Sue Goo ; Heo, Kyun ; Kim, In Hoo. / An RNA aptamer that specifically binds pancreatic adenocarcinoma up-regulated factor inhibits migration and growth of pancreatic cancer cells. In: Cancer Letters. 2011 ; Vol. 313, No. 1. pp. 76-83.
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abstract = "Previously, we reported that a novel secretory protein, pancreatic adenocarcinoma up-regulated factor (PAUF), which is highly expressed in pancreatic cancer and mediates the growth and metastasis of pancreatic cancer cells. In this study, we generated and characterized a 2'-fluoropyrimidine modified RNA aptamer (P12FR2) directed against human PAUF. P12FR2 binds specifically to human PAUF with an estimated apparent K D of 77. nM. P12FR2 aptamer inhibits PAUF-induced migration of PANC-1, human pancreatic cancer cells, in a wound healing assay. Moreover, intraperitoneal injection of P12FR2 decreased tumor growth by about 60{\%} in an in vivo xenograft model with CFPAC-1 pancreatic cancer cells, without causing a loss of weight in the treated mice. Taken together, we propose here that PAUF-specific RNA aptamer, P12FR2, has the potential to be effective in the therapy of human pancreatic cancer.",
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Kim, YH, Sung, HJ, Kim, S, Kim, EO, Lee, JW, Moon, JY, Choi, K, Jung, JE, Lee, Y, Koh, SS, Rhee, SG, Heo, K & Kim, IH 2011, 'An RNA aptamer that specifically binds pancreatic adenocarcinoma up-regulated factor inhibits migration and growth of pancreatic cancer cells', Cancer Letters, vol. 313, no. 1, pp. 76-83. https://doi.org/10.1016/j.canlet.2011.08.027

An RNA aptamer that specifically binds pancreatic adenocarcinoma up-regulated factor inhibits migration and growth of pancreatic cancer cells. / Kim, Yun Hee; Sung, Ho Jin; Kim, Sukyoung; Kim, Eun Ok; Lee, Ji Won; Moon, Ju Young; Choi, Kyungho; Jung, Ji Eun; Lee, Yangsoon; Koh, Sang Seok; Rhee, Sue Goo; Heo, Kyun; Kim, In Hoo.

In: Cancer Letters, Vol. 313, No. 1, 26.12.2011, p. 76-83.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Moon, Ju Young

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AU - Jung, Ji Eun

AU - Lee, Yangsoon

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AU - Heo, Kyun

AU - Kim, In Hoo

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