Amyloid beta peptide directly inhibits PKC activation

Woojin Lee, Jung Hyun Boo, Min Whan Jung, Sang Dai Park, Young Ho Kim, Seung U. Kim, Inhee Mook-Jung

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A putative protein kinase C (PKC) pseudosubstrate domain in beta amyloid (Aβ) suggests a potential interaction between Aβ and PKC. In this study, we investigated whether and how Aβ interacts with PKC. Aβ peptides inhibited PKC phosphorylation in a dose-dependent manner in cell-free in vitro condition, suggesting a direct interaction between Aβ and PKC. Experiments involving deletion of the Aβ sequence indicated that the putative PKC pseudosubstrate domain (Aβ 28-30) is critical for Aβ-PKC interaction. Addition of Aβ peptides to cultured B103 cells reduced the activated forms of PKCα and PKCε. It also inhibited phorbol-12,13-dibutyrate (PDBu)-induced membrane translocation of PKCα and PKCε without altering their expression levels, indicating that activation of intracellular PKC is inhibited by treatment of Aβ peptides. These results suggest that Aβ peptides inhibit PKC activation via direct interactions, which may play a role in pathogenesis of AD.

Original languageEnglish
Pages (from-to)222-231
Number of pages10
JournalMolecular and Cellular Neuroscience
Volume26
Issue number2
DOIs
StatePublished - 1 Jun 2004

Fingerprint

Amyloid beta-Peptides
Protein Kinase C
Peptides
Phorbol 12,13-Dibutyrate
Sequence Deletion
Amyloid
Cultured Cells
Membrane Proteins
Phosphorylation

Keywords

  • AD
  • APP
  • Alzheimer's disease
  • Beta amyloid
  • PKC
  • Phosphorylation
  • Protein kinase C
  • amyloid precursor protein
  • beta amyloid
  • protein kinase C

Cite this

Lee, W., Boo, J. H., Jung, M. W., Park, S. D., Kim, Y. H., Kim, S. U., & Mook-Jung, I. (2004). Amyloid beta peptide directly inhibits PKC activation. Molecular and Cellular Neuroscience, 26(2), 222-231. https://doi.org/10.1016/j.mcn.2003.10.020
Lee, Woojin ; Boo, Jung Hyun ; Jung, Min Whan ; Park, Sang Dai ; Kim, Young Ho ; Kim, Seung U. ; Mook-Jung, Inhee. / Amyloid beta peptide directly inhibits PKC activation. In: Molecular and Cellular Neuroscience. 2004 ; Vol. 26, No. 2. pp. 222-231.
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Amyloid beta peptide directly inhibits PKC activation. / Lee, Woojin; Boo, Jung Hyun; Jung, Min Whan; Park, Sang Dai; Kim, Young Ho; Kim, Seung U.; Mook-Jung, Inhee.

In: Molecular and Cellular Neuroscience, Vol. 26, No. 2, 01.06.2004, p. 222-231.

Research output: Contribution to journalArticle

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