Alternative splicing of gar-1, a Caenorhabditis elegans G-protein-linked acetylcholine receptor gene

Yang Seo Park, Yong-Seok Lee, Nam Jeong Cho, Bong Kiun Kaang

Research output: Contribution to journalArticleResearchpeer-review

10 Citations (Scopus)

Abstract

We have recently identified a gene, designated gar-1, coding for a novel form of G-protein-linked acetylcholine (ACh) receptor in Caenorhabditis elegans. Although this receptor is most closely related to muscarinic ACh receptors (mAChRs), electrophysiological analyses have shown that ligand binding specificity of the receptor is distinct from that of mAChRs. Here we report that three receptor isoforms are generated by alternative splicing of the gar-1 transcript. These receptor isoforms differ only in the third intracellular loop that is considered to be important for G protein coupling. The three splice variants, when expressed in Xenopus oocyte, displayed similar pharmacological profiles and signaling activities. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis showed that the three gar-1 mRNAs are present at all developmental stages examined. The results in this study provide evidence that alternative splicing is involved in promoting molecular diversity of G-protein-linked ACh receptors. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)354-358
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume268
Issue number2
DOIs
StatePublished - 16 Feb 2000

Fingerprint

Caenorhabditis elegans Proteins
Alternative Splicing
Cholinergic Receptors
GTP-Binding Proteins
Genes
Muscarinic Receptors
Protein Isoforms
Polymerase chain reaction
RNA-Directed DNA Polymerase
Caenorhabditis elegans
Xenopus
Reverse Transcriptase Polymerase Chain Reaction
Cholinergic Agents
Oocytes
Pharmacology
Ligands
Messenger RNA

Keywords

  • Alternative splicing
  • Caenorhabditis elegans
  • G-protein-linked acetylcholine receptor
  • gar-1

Cite this

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Alternative splicing of gar-1, a Caenorhabditis elegans G-protein-linked acetylcholine receptor gene. / Park, Yang Seo; Lee, Yong-Seok; Cho, Nam Jeong; Kaang, Bong Kiun.

In: Biochemical and Biophysical Research Communications, Vol. 268, No. 2, 16.02.2000, p. 354-358.

Research output: Contribution to journalArticleResearchpeer-review

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