Alterations in intracellular Ca2+ levels in human endometrial stromal cells after decidualization

Jie Ohn Sohn, Seung-Yong Seong, Hyun Jin Kim, Yoon Mi Jo, Kyoung Hoon Lee, Mi Kyung Chung, Hyun Jin Song, Kyoung Sun Park, Jeong Mook Lim

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Calcium (Ca2+) is an important element for many physiological functions of the uterus, including embryo implantation. Here, we investigated the possible involvement of altered intracellular Ca2+ levels in decidualization in human endometrial stromal cells (hEMSCs). hEMSCs showed high levels of mesenchymal stem cell marker expression (CD73, CD90, and CD105) and did not express markers of hematopoietic progenitor cells (CD31, CD34, CD45, and HLA-DR). Decidualization is a process of ovarian steroid-induced endometrial stromal cell proliferation and differentiation. Several types of ion channels, which are regulated by the ovarian hormones progesterone and estradiol, as well as growth factors, are important for endometrial receptivity and embryo implantation. The combined application of progesterone (1 μM medroxyprogesterone acetate) and cyclic AMP (0.5 mM) for 6 days not only elevated inositol 1,4,5-triphosphate receptor (IP3R)-mediated Ca2+ release and IP3R expression, it also promoted ORAI and STIM expression as well as cyclopiazonic acid-induced Ca2+ release. Finally, intracellular Ca2+ levels and ion channel gene expression influenced hEMSC proliferation. These results suggest that cytosolic Ca2+ dynamics, mediated by specific ion channels, serve as an important step in the decidualization of hEMSCs.

Original languageEnglish
Pages (from-to)318-324
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume515
Issue number2
DOIs
StatePublished - 23 Jul 2019

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Stromal Cells
Ion Channels
Cell proliferation
Progesterone
Inositol 1,4,5-Trisphosphate Receptors
Medroxyprogesterone Acetate
HLA-DR Antigens
Stem cells
Gene expression
Cyclic AMP
Cell Proliferation
Estradiol
Intercellular Signaling Peptides and Proteins
Steroids
Hormones
Calcium
Hematopoietic Stem Cells
Mesenchymal Stromal Cells
Uterus
Cell Differentiation

Keywords

  • Cytosolic Ca2+ regulation
  • Decidualization
  • Endometrial stromal cells
  • Ion channels
  • Proliferation

Cite this

Sohn, Jie Ohn ; Seong, Seung-Yong ; Kim, Hyun Jin ; Jo, Yoon Mi ; Lee, Kyoung Hoon ; Chung, Mi Kyung ; Song, Hyun Jin ; Park, Kyoung Sun ; Lim, Jeong Mook. / Alterations in intracellular Ca2+ levels in human endometrial stromal cells after decidualization. In: Biochemical and Biophysical Research Communications. 2019 ; Vol. 515, No. 2. pp. 318-324.
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Alterations in intracellular Ca2+ levels in human endometrial stromal cells after decidualization. / Sohn, Jie Ohn; Seong, Seung-Yong; Kim, Hyun Jin; Jo, Yoon Mi; Lee, Kyoung Hoon; Chung, Mi Kyung; Song, Hyun Jin; Park, Kyoung Sun; Lim, Jeong Mook.

In: Biochemical and Biophysical Research Communications, Vol. 515, No. 2, 23.07.2019, p. 318-324.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Sohn, Jie Ohn

AU - Seong, Seung-Yong

AU - Kim, Hyun Jin

AU - Jo, Yoon Mi

AU - Lee, Kyoung Hoon

AU - Chung, Mi Kyung

AU - Song, Hyun Jin

AU - Park, Kyoung Sun

AU - Lim, Jeong Mook

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