Alteration of pituitary tumor transforming gene 1 by microRNA-186 and 655 regulates invasion ability of human oral squamous cell carcinoma

Sang Shin Lee, Jong Ho Choi, Seung Mook Lim, Gi Jin Kim, Suk Keun Lee, Yoon Kyung Jeon

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Pituitary tumor-transforming gene 1 (PTTG1) was recently shown to be involved in the progression as well as the metastasis of cancers. However, their expression and function in the invasion of oral squamous cell carcinoma (SCC) remain unclear. Methods: The ex-pressions of PTTG1 and PTTG1-targeted miRNA in oral SCC cell lines and their invasion capability depended on PTTG1 expression were analyzed by quantitative RT-PCR, Western blots, the transwell insert system and Zymography. Results: Invasion abilities were decreased in oral SCC cells treated with siRNA-PTTG1. When PTTG1 were downregulated in oral SCC cells treated with microRNA-186 and-655 inhibited their invasion abilities via MMP-9 activity. Conclusions: These results indicate that alteration of expression of PTTG1 in oral SCC cells by newly identified mi-croRNA-186 and-655 can regulate invasion activity. Therefore, these data offer new insights into further understanding PTTG1 function in oral SCC and should provide new strategies for diagnostic markers for oral SCC.

Original languageEnglish
Article number1021
Pages (from-to)1-17
Number of pages17
JournalInternational Journal of Molecular Sciences
Volume22
Issue number3
DOIs
StatePublished - 1 Feb 2021

Keywords

  • Invasion
  • MicroRNA
  • Oral squamous cell carcinoma
  • Pituitary tumor-transforming gene 1

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