Adenosine stimulates anion secretion across cultured and native adult human vas deferens epithelia

Ryan W. Carlin, Jun Ho Lee, Daniel C. Marcus, Bruce D. Schultz

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Experiments were conducted to determine the responsiveness of human vas deferens epithelial cell monolayers to adenosine and related agonists. Human abdominal vas deferens epithelial cells have been isolated from adult tissues and grown to confluence on permeable supports. All cells exhibit intense ZO-1 and cytokeratin immunoreactivity. Cultured cell monolayers exhibit high electrical resistance with a lumen-negative potential difference and short circuit current (Isc) indicative of anion secretion and/or cation absorption. A portion of the basal Isc is inhibited by amiloride. Amiloride-sensitive Isc is enhanced by exposure to glucocorticoids and is Na+ dependent, indicating the presence of epithelial sodium channel-mediated Na+ absorption. Epithelial anion secretion and intracellular generation of cAMP are acutely stimulated by adenosine and the adenosine receptor agonist 5'-(N-ethylcarboxamido)adenosine (NECA), with these effects being fully blocked by 8-phenyltheophylline. Adenosine receptors are localized to the apical membrane of the epithelial cells, as basolateral adenosine is without effect. Freshly excised human vas deferens recapitulate observations made on cultured epithelia when evaluated with the self-referencing vibrating probe: amiloride inhibition of basal ion transport, stimulation by adenosine, and inhibition by 8-phenyltheophyline. These results demonstrate that adult human vas deferens epithelium actively transports ions to generate the luminal environment of the deferent duct. Thus, vas deferens epithelium likely plays an active role in male fertility, and interventions that modulate epithelial function might be exploited to treat male-factor infertility or in contraception.

Original languageEnglish
Pages (from-to)1027-1034
Number of pages8
JournalBiology of Reproduction
Volume68
Issue number3
DOIs
StatePublished - 1 Mar 2003

Fingerprint

Vas Deferens
Population Groups
Adenosine
Anions
Epithelium
Amiloride
Epithelial Cells
Ion Transport
Purinergic P1 Receptor Agonists
Epithelial Sodium Channels
Purinergic P1 Receptors
Male Infertility
Keratins
Electric Impedance
Contraception
Glucocorticoids
Fertility
Cations
Cultured Cells
Membranes

Keywords

  • Cortisol
  • Male reproductive tract
  • Male sexual function
  • Neuroendocrinology
  • Vas deferens

Cite this

Carlin, Ryan W. ; Lee, Jun Ho ; Marcus, Daniel C. ; Schultz, Bruce D. / Adenosine stimulates anion secretion across cultured and native adult human vas deferens epithelia. In: Biology of Reproduction. 2003 ; Vol. 68, No. 3. pp. 1027-1034.
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Adenosine stimulates anion secretion across cultured and native adult human vas deferens epithelia. / Carlin, Ryan W.; Lee, Jun Ho; Marcus, Daniel C.; Schultz, Bruce D.

In: Biology of Reproduction, Vol. 68, No. 3, 01.03.2003, p. 1027-1034.

Research output: Contribution to journalArticle

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