Aberrant CpG island hypermethylation in pediatric gastric mucosa in association with helicobacter pylori infection

So Hyun Shin, Seog Yun Park, Jae Sung Ko, Nayoung Kim, Gyeong Hoon Kang

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Context.-Helicobacter pylori infection is primarily acquired during childhood and persists throughout life in the absence of eradication with antibiotics. Helicobacter pylori infection induces methylation in the promoter CpG island loci in gastric epithelial cells. Thus, aberrant CpG island hypermethylation in gastric epithelial cells likely occurs early in life, although there are no existing data supporting this notion. Objectives.-To identify whether aberrant CpG island hypermethylation occurs in pediatric stomach mucosa in association with H pylori infection and to compare methylation profiles of samples from pediatric and adult stomach tissues. Design.-We analyzed pediatric (n = 47) and adult (n = 38) gastric mucosa samples for their methylation status in 12 promoter CpG island loci using the MethyLight assay and compared the number of methylated genes and the methylation leveis in individual genes between H pyloripositive and H pylori-negative sample results and between pediatric and adult samples. Results.-The average number of methylated genes was significantly higher in H pylori-infected pediatric samples than in H pylori-negative pediatric samples (3.4 versus 0.3, P < .001) and in H pylori-infected adult samples than in H pylori-negative adult samples (7.6 versus 0.9, P < .001). Seven genes showed significantly higher methylation levels in H pylori-infected pediatric samples than in H pylorinegative pediatric samples (all values were P < .05). Conclusions.-These results indicate that CpG island hypermethylation occurs in pediatric gastric mucosa in association with H pylori infection and that the genes affected by H pylori-associated hypermethylation were similar in pediatric and adult samples.

Original languageEnglish
Pages (from-to)759-765
Number of pages7
JournalArchives of Pathology and Laboratory Medicine
Volume135
Issue number6
StatePublished - 1 Jun 2011

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