A transducible nuclear/nucleolar protein, mLLP, regulates neuronal morphogenesis and synaptic transmission

Nam Kyung Yu, Hyoung F. Kim, Jaehoon Shim, Somi Kim, Dae Won Kim, Chuljung Kwak, Su Eon Sim, Jun Hyeok Choi, Seohee Ahn, Juyoun Yoo, Sun Lim Choi, Deok Jin Jang, Chae Seok Lim, Yong-Seok Lee, Chulhun Kang, Soo Young Choi, Bong Kiun Kaang

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Abstract

Cell-permeable proteins are emerging as unconventional regulators of signal transduction and providing a potential for therapeutic applications. However, only a few of them are identified and studied in detail. We identify a novel cell-permeable protein, mouse LLP homolog (mLLP), and uncover its roles in regulating neural development. We found that mLLP is strongly expressed in developing nervous system and that mLLP knockdown or overexpression during maturation of cultured neurons affected the neuronal growth and synaptic transmission. Interestingly, extracellular addition of mLLP protein enhanced dendritic arborization, demonstrating the non-cell-autonomous effect of mLLP. Moreover, mLLP interacts with CCCTC-binding factor (CTCF) as well as transcriptional machineries and modulates gene expression involved in neuronal growth. Together, these results illustrate the characteristics and roles of previously unknown cell-permeable protein mLLP in modulating neural development.

Original languageEnglish
Article number22892
JournalScientific Reports
Volume6
DOIs
StatePublished - 10 Mar 2016

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Nuclear Proteins
Morphogenesis
Synaptic Transmission
Proteins
Neurologic Mutant Mice
Neuronal Plasticity
Growth
Nervous System
Signal Transduction
Gene Expression
Neurons

Cite this

Yu, N. K., Kim, H. F., Shim, J., Kim, S., Kim, D. W., Kwak, C., ... Kaang, B. K. (2016). A transducible nuclear/nucleolar protein, mLLP, regulates neuronal morphogenesis and synaptic transmission. Scientific Reports, 6, [22892]. https://doi.org/10.1038/srep22892
Yu, Nam Kyung ; Kim, Hyoung F. ; Shim, Jaehoon ; Kim, Somi ; Kim, Dae Won ; Kwak, Chuljung ; Sim, Su Eon ; Choi, Jun Hyeok ; Ahn, Seohee ; Yoo, Juyoun ; Choi, Sun Lim ; Jang, Deok Jin ; Lim, Chae Seok ; Lee, Yong-Seok ; Kang, Chulhun ; Choi, Soo Young ; Kaang, Bong Kiun. / A transducible nuclear/nucleolar protein, mLLP, regulates neuronal morphogenesis and synaptic transmission. In: Scientific Reports. 2016 ; Vol. 6.
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abstract = "Cell-permeable proteins are emerging as unconventional regulators of signal transduction and providing a potential for therapeutic applications. However, only a few of them are identified and studied in detail. We identify a novel cell-permeable protein, mouse LLP homolog (mLLP), and uncover its roles in regulating neural development. We found that mLLP is strongly expressed in developing nervous system and that mLLP knockdown or overexpression during maturation of cultured neurons affected the neuronal growth and synaptic transmission. Interestingly, extracellular addition of mLLP protein enhanced dendritic arborization, demonstrating the non-cell-autonomous effect of mLLP. Moreover, mLLP interacts with CCCTC-binding factor (CTCF) as well as transcriptional machineries and modulates gene expression involved in neuronal growth. Together, these results illustrate the characteristics and roles of previously unknown cell-permeable protein mLLP in modulating neural development.",
author = "Yu, {Nam Kyung} and Kim, {Hyoung F.} and Jaehoon Shim and Somi Kim and Kim, {Dae Won} and Chuljung Kwak and Sim, {Su Eon} and Choi, {Jun Hyeok} and Seohee Ahn and Juyoun Yoo and Choi, {Sun Lim} and Jang, {Deok Jin} and Lim, {Chae Seok} and Yong-Seok Lee and Chulhun Kang and Choi, {Soo Young} and Kaang, {Bong Kiun}",
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Yu, NK, Kim, HF, Shim, J, Kim, S, Kim, DW, Kwak, C, Sim, SE, Choi, JH, Ahn, S, Yoo, J, Choi, SL, Jang, DJ, Lim, CS, Lee, Y-S, Kang, C, Choi, SY & Kaang, BK 2016, 'A transducible nuclear/nucleolar protein, mLLP, regulates neuronal morphogenesis and synaptic transmission', Scientific Reports, vol. 6, 22892. https://doi.org/10.1038/srep22892

A transducible nuclear/nucleolar protein, mLLP, regulates neuronal morphogenesis and synaptic transmission. / Yu, Nam Kyung; Kim, Hyoung F.; Shim, Jaehoon; Kim, Somi; Kim, Dae Won; Kwak, Chuljung; Sim, Su Eon; Choi, Jun Hyeok; Ahn, Seohee; Yoo, Juyoun; Choi, Sun Lim; Jang, Deok Jin; Lim, Chae Seok; Lee, Yong-Seok; Kang, Chulhun; Choi, Soo Young; Kaang, Bong Kiun.

In: Scientific Reports, Vol. 6, 22892, 10.03.2016.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - A transducible nuclear/nucleolar protein, mLLP, regulates neuronal morphogenesis and synaptic transmission

AU - Yu, Nam Kyung

AU - Kim, Hyoung F.

AU - Shim, Jaehoon

AU - Kim, Somi

AU - Kim, Dae Won

AU - Kwak, Chuljung

AU - Sim, Su Eon

AU - Choi, Jun Hyeok

AU - Ahn, Seohee

AU - Yoo, Juyoun

AU - Choi, Sun Lim

AU - Jang, Deok Jin

AU - Lim, Chae Seok

AU - Lee, Yong-Seok

AU - Kang, Chulhun

AU - Choi, Soo Young

AU - Kaang, Bong Kiun

PY - 2016/3/10

Y1 - 2016/3/10

N2 - Cell-permeable proteins are emerging as unconventional regulators of signal transduction and providing a potential for therapeutic applications. However, only a few of them are identified and studied in detail. We identify a novel cell-permeable protein, mouse LLP homolog (mLLP), and uncover its roles in regulating neural development. We found that mLLP is strongly expressed in developing nervous system and that mLLP knockdown or overexpression during maturation of cultured neurons affected the neuronal growth and synaptic transmission. Interestingly, extracellular addition of mLLP protein enhanced dendritic arborization, demonstrating the non-cell-autonomous effect of mLLP. Moreover, mLLP interacts with CCCTC-binding factor (CTCF) as well as transcriptional machineries and modulates gene expression involved in neuronal growth. Together, these results illustrate the characteristics and roles of previously unknown cell-permeable protein mLLP in modulating neural development.

AB - Cell-permeable proteins are emerging as unconventional regulators of signal transduction and providing a potential for therapeutic applications. However, only a few of them are identified and studied in detail. We identify a novel cell-permeable protein, mouse LLP homolog (mLLP), and uncover its roles in regulating neural development. We found that mLLP is strongly expressed in developing nervous system and that mLLP knockdown or overexpression during maturation of cultured neurons affected the neuronal growth and synaptic transmission. Interestingly, extracellular addition of mLLP protein enhanced dendritic arborization, demonstrating the non-cell-autonomous effect of mLLP. Moreover, mLLP interacts with CCCTC-binding factor (CTCF) as well as transcriptional machineries and modulates gene expression involved in neuronal growth. Together, these results illustrate the characteristics and roles of previously unknown cell-permeable protein mLLP in modulating neural development.

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