A synthetic peptide blocking TRPV1 activation inhibits UV-induced skin responses

So Min Kang, Sangbum Han, Jang Hee Oh, Young Mee Lee, Chi Hyun Park, Chang Yup Shin, Dong Hun Lee, Jin Ho Chung

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Background Transient receptor potential type 1 (TRPV1) can be activated by ultraviolet (UV) irradiation, and mediates UV-induced matrix metalloproteinase (MMP)-1 and proinflammatory cytokines in keratinocytes. Various chemicals and compounds targeting TRPV1 activation have been developed, but are not in clinical use mostly due to their safety issues. Objective We aimed to develop a novel TRPV1-targeting peptide to inhibit UV-induced responses in human skin. Methods We designed and generated a novel TRPV1 inhibitory peptide (TIP) which mimics the specific site in TRPV1 (aa 701-709: Gln-Arg-Ala-Ile-Thr-Ile-Leu-Asp-Thr, QRAITILDT), Thr705, and tested its efficacy of blocking UV-induced responses in HaCaT, mouse, and human skin. Results TIP effectively inhibited capsaicin-induced calcium influx and TRPV1 activation. Treatment of HaCaT with TIP prevented UV-induced increases of MMP-1 and pro-inflammatory cytokines such as interleukin (IL)-6 and tumor necrosis factor-α. In mouse skin in vivo, TIP inhibited UV-induced skin thickening and prevented UV-induced expression of MMP-13 and MMP-9. Moreover, TIP attenuated UV-induced erythema and the expression of MMP-1, MMP-2, IL-6, and IL-8 in human skin in vivo. Conclusion The novel synthetic peptide targeting TRPV1 can ameliorate UV-induced skin responses in vitro and in vivo, providing a promising therapeutic approach against UV-induced inflammation and photoaging.

Original languageEnglish
Pages (from-to)126-133
Number of pages8
JournalJournal of Dermatological Science
Volume88
Issue number1
DOIs
StatePublished - Oct 2017

Keywords

  • Matrix metalloproteinases
  • Pro-inflammatory cytokines
  • Transient receptor potential vanilloid 1
  • Ultraviolet

Cite this