A recombinant mouse GM-CSF protein expressed as an inclusion form shows colony stimulating activity

Jin Kyoo Kim, Eun Jung Sohn, Lee Soo-O, Choon Taek Lee, Lee Ah Young, Hye Kyung Chung, Bong Whan Sung, Hyun Joo Youn

Research output: Contribution to journalArticleResearchpeer-review

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Abstract

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a pleiotropic hematopoietic growth factor and an activator of mature myeloid cells, and recombinant GM-CSF is increasingly under clinical studies for the treatment of various diseases including cancer, infectious diseases and hematopoietic diseases. We constructed a recombinant mouse GM-CSF expression plasmid with pelB leader sequence and His.Tag under T7 promoter control, and showed that the construct produced a 20 kDa recombinant protein in 8M urea. We also showed that the 20 kDa recombinant protein prepared in 8M urea stimulated colony formation in vitro, indicating that the recombinant mGM-CSF can be renatured to its native form to show the colony stimulating activity.

Original languageEnglish
Pages (from-to)109-112
Number of pages4
JournalJournal of Microbiology
Volume38
Issue number2
StatePublished - 1 Dec 2000

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Granulocyte-Macrophage Colony-Stimulating Factor
Recombinant Proteins
Urea
Proteins
Myeloid Cells
Communicable Diseases
Intercellular Signaling Peptides and Proteins
Plasmids
Neoplasms

Keywords

  • Colony stimulating activity
  • Expression construct
  • GM-CSF
  • His-Tag
  • Recombinant protein

Cite this

Kim, J. K., Sohn, E. J., Soo-O, L., Lee, C. T., Ah Young, L., Chung, H. K., ... Youn, H. J. (2000). A recombinant mouse GM-CSF protein expressed as an inclusion form shows colony stimulating activity. Journal of Microbiology, 38(2), 109-112.
Kim, Jin Kyoo ; Sohn, Eun Jung ; Soo-O, Lee ; Lee, Choon Taek ; Ah Young, Lee ; Chung, Hye Kyung ; Sung, Bong Whan ; Youn, Hyun Joo. / A recombinant mouse GM-CSF protein expressed as an inclusion form shows colony stimulating activity. In: Journal of Microbiology. 2000 ; Vol. 38, No. 2. pp. 109-112.
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abstract = "Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a pleiotropic hematopoietic growth factor and an activator of mature myeloid cells, and recombinant GM-CSF is increasingly under clinical studies for the treatment of various diseases including cancer, infectious diseases and hematopoietic diseases. We constructed a recombinant mouse GM-CSF expression plasmid with pelB leader sequence and His.Tag under T7 promoter control, and showed that the construct produced a 20 kDa recombinant protein in 8M urea. We also showed that the 20 kDa recombinant protein prepared in 8M urea stimulated colony formation in vitro, indicating that the recombinant mGM-CSF can be renatured to its native form to show the colony stimulating activity.",
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Kim, JK, Sohn, EJ, Soo-O, L, Lee, CT, Ah Young, L, Chung, HK, Sung, BW & Youn, HJ 2000, 'A recombinant mouse GM-CSF protein expressed as an inclusion form shows colony stimulating activity', Journal of Microbiology, vol. 38, no. 2, pp. 109-112.

A recombinant mouse GM-CSF protein expressed as an inclusion form shows colony stimulating activity. / Kim, Jin Kyoo; Sohn, Eun Jung; Soo-O, Lee; Lee, Choon Taek; Ah Young, Lee; Chung, Hye Kyung; Sung, Bong Whan; Youn, Hyun Joo.

In: Journal of Microbiology, Vol. 38, No. 2, 01.12.2000, p. 109-112.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Kim, Jin Kyoo

AU - Sohn, Eun Jung

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AU - Ah Young, Lee

AU - Chung, Hye Kyung

AU - Sung, Bong Whan

AU - Youn, Hyun Joo

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AB - Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a pleiotropic hematopoietic growth factor and an activator of mature myeloid cells, and recombinant GM-CSF is increasingly under clinical studies for the treatment of various diseases including cancer, infectious diseases and hematopoietic diseases. We constructed a recombinant mouse GM-CSF expression plasmid with pelB leader sequence and His.Tag under T7 promoter control, and showed that the construct produced a 20 kDa recombinant protein in 8M urea. We also showed that the 20 kDa recombinant protein prepared in 8M urea stimulated colony formation in vitro, indicating that the recombinant mGM-CSF can be renatured to its native form to show the colony stimulating activity.

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