A bioprinted human-glioblastoma-on-a-chip for the identification of patient-specific responses to chemoradiotherapy

Hee Gyeong Yi, Young Hun Jeong, Yona Kim, Yeong Jin Choi, Hyo Eun Moon, Sung Hye Park, Kyung Shin Kang, Mihyeon Bae, Jinah Jang, Hyewon Youn, Sun Ha Paek, Dong Woo Cho

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Patient-specific ex vivo models of human tumours that recapitulate the pathological characteristics and complex ecology of native tumours could help determine the most appropriate cancer treatment for individual patients. Here, we show that bioprinted reconstituted glioblastoma tumours consisting of patient-derived tumour cells, vascular endothelial cells and decellularized extracellular matrix from brain tissue in a compartmentalized cancer–stroma concentric-ring structure that sustains a radial oxygen gradient, recapitulate the structural, biochemical and biophysical properties of the native tumours. We also show that the glioblastoma-on-a-chip reproduces clinically observed patient-specific resistances to treatment with concurrent chemoradiation and temozolomide, and that the model can be used to determine drug combinations associated with superior tumour killing. The patient-specific tumour-on-a-chip model might be useful for the identification of effective treatments for glioblastoma patients resistant to the standard first-line treatment.

Original languageEnglish
Pages (from-to)509-519
Number of pages11
JournalNature Biomedical Engineering
Volume3
Issue number7
DOIs
StatePublished - 1 Jul 2019

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Chemoradiotherapy
Glioblastoma
Tumors
Neoplasms
temozolomide
Oncology
Endothelial cells
Drug Combinations
Ecology
Therapeutics
Brain
Identification (control systems)
Extracellular Matrix
Cells
Tissue
Oxygen
Endothelial Cells

Cite this

Yi, Hee Gyeong ; Jeong, Young Hun ; Kim, Yona ; Choi, Yeong Jin ; Moon, Hyo Eun ; Park, Sung Hye ; Kang, Kyung Shin ; Bae, Mihyeon ; Jang, Jinah ; Youn, Hyewon ; Paek, Sun Ha ; Cho, Dong Woo. / A bioprinted human-glioblastoma-on-a-chip for the identification of patient-specific responses to chemoradiotherapy. In: Nature Biomedical Engineering. 2019 ; Vol. 3, No. 7. pp. 509-519.
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A bioprinted human-glioblastoma-on-a-chip for the identification of patient-specific responses to chemoradiotherapy. / Yi, Hee Gyeong; Jeong, Young Hun; Kim, Yona; Choi, Yeong Jin; Moon, Hyo Eun; Park, Sung Hye; Kang, Kyung Shin; Bae, Mihyeon; Jang, Jinah; Youn, Hyewon; Paek, Sun Ha; Cho, Dong Woo.

In: Nature Biomedical Engineering, Vol. 3, No. 7, 01.07.2019, p. 509-519.

Research output: Contribution to journalArticle

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