Aim:To investigate the effect of -shogaol, an active ingredient in ginger, on melanogenesis and the underlying mechanisms. Methods:B16F10 mouse melanoma cells were tested. Cell viability was determined with the MTT assay. Melanin content and tyrosinase activity were analyzed with a spectrophotometer. The protein expression of tyrosinase and microphthalmia associated transcription factor (MITF), as well as phosphorylated or total ERK1/2 and Akt were measured using Western blot. Results:Treatment of the cells with -shogaol (1, 5, 10 μmol/L) reduced the melanin content in a concentration-dependent manner. -Shogaol (5 and 10 μmol/L) significantly decreased the intracellular tyrosinase activity, and markedly suppressed the expression levels of tyrosinase and MITF proteins in the cells. Furthermore, -shogaol (10 μmol/L) activated ERK, which was known to negatively regulate melanin synthesis in these cells. Pretreatment with the specific ERK pathway inhibitor PD98059 (20 μmol/L) greatly attenuated the inhibition of melanin synthesis by -shogaol (10 μmol/L). Conclusion:The results demonstrate that -shogaol inhibits melanogenesis in B16F10 mouse melanoma cells via activating the ERK pathway.
- B16F10 mouse melanoma cell
- skin pigmentation