α-tocopherol transfer protein expression in rat liver exposed to hyperoxia

Ryoichi Ban, Kimitaka Takitani, Han Suk Kim, Takuji Murata, Takao Morinobu, Tohru Ogihara, Hiroshi Tamai

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

α-Tochopherol transfer protein (αTTP), a 32kDa protein exclusively expressed in liver cytosol, has a high binding affinity for α-tochopherol. The factors that regulate the expression of hepatic αTTP are not clearly understood. In this study, we investigated whether or not exposure to hyperoxia (>95% O2 for 48h) could alter the expression of hepatic αTTP. We also examined the association between the expression of antioxidant enzymes (hepatic glutathione peroxidase (GPX) and Mn-superoxide dismutase (Mn-SOD)) and the expression of hepatic αTTP. The levels of thiobarbituric acid-reactive substances (TBARS) in both plasma and liver were significantly higher after rats were exposed to hyperoxia for 48 h when compared with the levels in control rats. Northern blotting showed a decrease in the expression of αTTP messenger RNA (mRNA) after hyperoxia, although the αTTP protein level remained constant. Expression of Mn-SOD mRNA and protein, as well as the expression of GPX mRNA, were stable after hyperoxia. These findings indicate that mRNA for hepatic αTTP, rather than Mn-SOD or GPX, may be highly responsive to oxidative stress.

Original languageEnglish
Pages (from-to)933-938
Number of pages6
JournalFree Radical Research
Volume36
Issue number9
DOIs
StatePublished - 20 Aug 2002

Fingerprint

Hyperoxia
Tocopherols
Liver
Rats
Proteins
Glutathione Peroxidase
Superoxide Dismutase
Messenger RNA
Rat control
Oxidative stress
Thiobarbituric Acid Reactive Substances
Northern Blotting
Cytosol
Oxidative Stress
Antioxidants
Association reactions
Plasmas

Keywords

  • Glutathione peroxidase
  • Hyperoxia
  • Mn-superoxide dismutase
  • α-tocopherol
  • α-tocopherol transfer protein

Cite this

Ban, R., Takitani, K., Kim, H. S., Murata, T., Morinobu, T., Ogihara, T., & Tamai, H. (2002). α-tocopherol transfer protein expression in rat liver exposed to hyperoxia. Free Radical Research, 36(9), 933-938. https://doi.org/10.1080/107156021000006644
Ban, Ryoichi ; Takitani, Kimitaka ; Kim, Han Suk ; Murata, Takuji ; Morinobu, Takao ; Ogihara, Tohru ; Tamai, Hiroshi. / α-tocopherol transfer protein expression in rat liver exposed to hyperoxia. In: Free Radical Research. 2002 ; Vol. 36, No. 9. pp. 933-938.
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Ban, R, Takitani, K, Kim, HS, Murata, T, Morinobu, T, Ogihara, T & Tamai, H 2002, 'α-tocopherol transfer protein expression in rat liver exposed to hyperoxia', Free Radical Research, vol. 36, no. 9, pp. 933-938. https://doi.org/10.1080/107156021000006644

α-tocopherol transfer protein expression in rat liver exposed to hyperoxia. / Ban, Ryoichi; Takitani, Kimitaka; Kim, Han Suk; Murata, Takuji; Morinobu, Takao; Ogihara, Tohru; Tamai, Hiroshi.

In: Free Radical Research, Vol. 36, No. 9, 20.08.2002, p. 933-938.

Research output: Contribution to journalArticle

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AU - Ogihara, Tohru

AU - Tamai, Hiroshi

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